Guccione Clizia, Bergonzi Maria Camilla, Piazzini Vieri, Bilia Anna Rita
University of Florence, Department of Chemistry, Sesto Fiorentino, Via Ugo Schiff 6, 50019, Florence, Italy.
Planta Med. 2016 Jul;82(11-12):1039-45. doi: 10.1055/s-0042-108735. Epub 2016 Jun 9.
A chromatographic method for the qualitative and quantitative characterization of peels and preparations based on different species of Citrus was developed in order to obtain a complete profile of the constituents, including flavonoids and protoalkaloids. Commercial peels of sweet orange, lemon, mandarin, and grapefruit were analyzed. Seventeen constituents including flavanones, flavones, polymethoxyflavones, and protoalkaloids were identified by HPLC-PDA, HPLC-MS, and HPLC-MS/MS using a comparison of retention times and UV-Vis and MS spectra with reference standards and literature data. The total amount of flavanones [neoeriocitrin (5), naringin (8) and hesperidin (9)] and polymethoxflavones [sinensetin (12), nobiletin (14), 3,5,6,7,8,3',4'-heptamethoxyflavone (15), and tangeretin (16)] was determined and expressed as naringin (8) or hesperidin (9), and sinensetin (12), respectively. The protoalkaloid synephrine was detected in all samples, except in grapefruit, but its content was lower than the limit of quantification. Qualitative and quantitative chemical profiles of three different Italian aromatic liquors ("Limoncello", "Arancello", and "Mandarinetto"), prepared according to traditional recipes, were also analyzed.
开发了一种色谱方法，用于对基于不同柑橘品种的果皮及其制剂进行定性和定量表征，以获得包括黄酮类化合物和原生物碱在内的成分的完整概况。对甜橙、柠檬、柑橘和葡萄柚的市售果皮进行了分析。通过HPLC-PDA、HPLC-MS和HPLC-MS/MS，利用保留时间以及紫外可见光谱和质谱与参考标准品及文献数据的比较，鉴定出了17种成分，包括黄烷酮、黄酮、多甲氧基黄酮和原生物碱。测定了黄烷酮[新橙皮苷（5）、柚皮苷（8）和橙皮苷（9）]和多甲氧基黄酮[川陈皮素（12）、诺米林（14）、3,5,6,7,8,3',4'-七甲氧基黄酮（15）和橘皮素（16）]的总量，并分别以柚皮苷（8）或橙皮苷（9）以及川陈皮素（12）表示。除葡萄柚外，在所有样品中均检测到了原生物碱辛弗林，但其含量低于定量限。还分析了按照传统配方制备的三种不同意大利芳香酒（“柠檬甜酒”、“橙子甜酒”和“柑橘甜酒”）的定性和定量化学概况。
