College of Food Science and Engineering, Qingdao Agricultural University, Qingdao 266109, PR China.
College of Food Science and Engineering, Qingdao Agricultural University, Qingdao 266109, PR China.
Carbohydr Polym. 2014 Nov 26;113:314-24. doi: 10.1016/j.carbpol.2014.07.025. Epub 2014 Jul 21.
An efficient ultrasound-assisted enzymatic extraction (UAEE) of Cucurbita moschata polysaccharides (CMCP) was established and the CMCP antioxidant activities were studied. The UAEE operating parameters (extraction temperature, ultrasonic power, pH, and liquid-to-material ratio) were optimized using the central composite design (CCD) and the mass transfer kinetic study in UAEE procedure was used to select the optimal extraction time. Enzymolysis and ultrasonication that were simultaneously conducted was selected as the UAEE synergistic model and the optimum extraction conditions with a maximum polysaccharide yield of 4.33 ± 0.15% were as follows: extraction temperature, 51.5 °C; ultrasonic power, 440 W; pH, 5.0; liquid-to-material ratio, 5.70:1 mL/g; and extraction time, 20 min. Evaluation of the antioxidant activity in vitro suggested that CMCP has good potential as a natural antioxidant used in the food or medicine industry because of their high reducing power and positive radical scavenging activity for DPPH radical.
建立了一种高效的超声辅助酶解提取（UAEE）南瓜多糖（CMCP）的方法，并研究了 CMCP 的抗氧化活性。采用中心组合设计（CCD）优化了 UAEE 的操作参数（提取温度、超声功率、pH 值和液料比），并在 UAEE 过程中进行传质动力学研究，以选择最佳的提取时间。选择同时进行酶解和超声处理的 UAEE 协同模型，最佳提取条件为多糖得率最高，为 4.33±0.15%，具体条件为：提取温度 51.5°C，超声功率 440 W，pH 值 5.0，液料比 5.70:1 mL/g，提取时间 20 min。体外抗氧化活性评价表明，CMCP 具有作为食品或医药工业用天然抗氧化剂的潜力，因为其具有较高的还原能力和对 DPPH 自由基的正清除活性。
