Kan Yongjun, Chen Tiqiang, Wu Yanbin, Wu Jianguo, Wu Jinzhong
Pharmacy College, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, PR China.
Institute of Edible Fungi, Fujian Academy of Agricultural Sciences, Fuzhou 350013, PR China.
Int J Biol Macromol. 2015 Jan;72:151-7. doi: 10.1016/j.ijbiomac.2014.07.056. Epub 2014 Aug 19.
Superfine grinding technology was applied for polysaccharide extraction from the fruiting bodies of Ganoderma lucidum, and response surface methodology (RSM) was used to optimize the effects of processing parameters on polysaccharide extraction yield. Results showed that the maximum yield of G. lucidum polysaccharides (GLP) was obtained at an optimum condition: extraction time 137 min, extraction temperature 66 ̊C, the ratio of water to material 35 mL/g, and the GLP extracting yield reached 2.44% under this condition. GLP were precipitated into three crude polysaccharides, viz. GLP40, GLP60 and GLP80. The basic characterization of polysaccharides was determined by using HPLC and FT-IR methods. GLP, GLP80, GLP60, and GLP40 were composed of Man, Rib, Glc, Gal and Fuc with the molar ratios of 1.27:0.36:22.89:1.61:0.33, 1.40:0.31:23.02:3.46:0.91, 0.96:0.34:25.76:2.47:0.46, and 2.81:1.42:23.83:1.61:0.33, respectively. The result of FT-IR suggested that the monosaccharide residue of the four polysaccharides was β-pyranoid ring. Moreover, the antioxidant activities of these four polysaccharides were evaluated. The results showed that GLP80 had the best reducing power, DPPH radical scavenging ability and oxygen radical scavenging ability followed by GLP, GLP60 and GLP40. Our results demonstrated that RSM might be a valuable technique for optimizing the efficient extraction of GLP, and G. lucidum could be considered as sources of natural antioxidants and preservatives of food industry. Moreover, polysaccharides, especially GLP80, extracted from the fruiting bodies of G. lucidum, exhibited promising antioxidant activities.
采用超微粉碎技术从灵芝子实体中提取多糖，并运用响应面法（RSM）优化工艺参数对多糖提取率的影响。结果表明，在最佳条件下可获得灵芝多糖（GLP）的最大产量：提取时间137分钟，提取温度66℃，料液比35 mL/g，在此条件下GLP提取率达到2.44%。GLP沉淀为三种粗多糖，即GLP40、GLP60和GLP80。采用高效液相色谱（HPLC）和傅里叶变换红外光谱（FT-IR）方法对多糖进行基本表征。GLP、GLP80、GLP60和GLP40由甘露糖（Man）、核糖（Rib）、葡萄糖（Glc）、半乳糖（Gal）和岩藻糖（Fuc）组成，摩尔比分别为1.27:0.36:22.89:1.61:0.33、1.40:0.31:23.02:3.46:0.91、0.96:0.34:25.76:2.47:0.46和2.81:1.42:23.83:1.61:0.33。FT-IR结果表明，四种多糖的单糖残基均为β-吡喃环。此外，对这四种多糖的抗氧化活性进行了评价。结果表明，GLP80具有最佳的还原力、DPPH自由基清除能力和氧自由基清除能力，其次是GLP、GLP60和GLP40。我们的结果表明，响应面法可能是优化GLP高效提取的一种有价值的技术，灵芝可被视为食品工业天然抗氧化剂和防腐剂的来源。此外，从灵芝子实体中提取的多糖，尤其是GLP80，表现出良好的抗氧化活性。
