Liu Xiao Tong, Mu Xi Yan, Wu Xiao Li, Meng Li Xuan, Guan Wen Bi, Ma Yong Qiang, Sun Hua, Wang Cheng Ju, Li Xue Feng
College of Science, China Agricultural University, Beijing 100193, China.
College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China.
Biomed Environ Sci. 2014 Sep;27(9):676-83. doi: 10.3967/bes2014.103.
This study was aimed to investigate the toxic effects of 3 nanomaterials, i.e. multi-walled carbon nanotubes (MWCNTs), graphene oxide (GO), and reduced graphene oxide (RGO), on zebrafish embryos.
The 2-h post-fertilization (hpf) zebrafish embryos were exposed to MWCNTs, GO, and RGO at different concentrations (1, 5, 10, 50, 100 mg/L) for 96 h. Afterwards, the effects of the 3 nanomateria on spontaneous movement, heart rate, hatching rate, length of larvae, mortality, and malformations ls were evaluated.
Statistical analysis indicated that RGO significantly inhibited the hatching of zebrafish embryos. Furthermore, RGO and MWCNTs decreased the length of the hatched larvae at 96 hpf. No obvious morphological malformation or mortality was observed in the zebrafish embryos after exposure to the three nanomaterials.
MWCNTs, GO, and RGO were all toxic to zebrafish embryos to influence embryos hatching and larvae length. Although no obvious morphological malformation and mortality were observed in exposed zebrafish embryos, further studies on the toxicity of the three nanomaterials are still needed.
本研究旨在探究3种纳米材料，即多壁碳纳米管（MWCNTs）、氧化石墨烯（GO）和还原氧化石墨烯（RGO）对斑马鱼胚胎的毒性作用。
将受精后2小时（hpf）的斑马鱼胚胎暴露于不同浓度（1、5、10、50、100 mg/L）的MWCNTs、GO和RGO中96小时。之后，评估这3种纳米材料对自发运动、心率、孵化率、幼虫长度、死亡率和畸形的影响。
统计分析表明，RGO显著抑制斑马鱼胚胎的孵化。此外，RGO和MWCNTs在96 hpf时缩短了孵化后幼虫的长度。暴露于这三种纳米材料后，斑马鱼胚胎未观察到明显的形态畸形或死亡。
MWCNTs、GO和RGO对斑马鱼胚胎均有毒性，影响胚胎孵化和幼虫长度。尽管在暴露的斑马鱼胚胎中未观察到明显的形态畸形和死亡，但仍需要对这三种纳米材料的毒性进行进一步研究。
