粗糙脉孢菌对DNA损伤或应激诱导剂的诱导反应。

Inducible responses to DNA damaging or stress inducing agents in Neurospora crassa.

作者信息

Howard C A, Baker T I

机构信息

Department of Microbiology, School of Medicine, University of New Mexico, Albuquerque 87131.

出版信息

Curr Genet. 1989 Jan;15(1):47-55. doi: 10.1007/BF00445751.

DOI:10.1007/BF00445751

PMID:2525961

Abstract

Two-dimensional polyacrylamide gel electrophoresis has been used to analyze proteins from wild type and mutagen sensitive mutants of Neurospora crassa under constitutive conditions and after treatment with mutagens and other stress inducing agents. Several proteins have been detected that are either induced or show changes in electrophoretic mobility in response to UV irradiation, 4-NQO, X-ray, paraquat and heat shock. Ten proteins were found to respond to more than one of the stress inducing agents, demonstrating a complex pattern of polypeptide inductions. The significance of these findings and the possible effects of some of these proteins on the DNA repair process and stress management are discussed.

摘要

二维聚丙烯酰胺凝胶电泳已被用于分析野生型和对诱变剂敏感的粗糙脉孢菌突变体在组成型条件下以及用诱变剂和其他应激诱导剂处理后的蛋白质。已检测到几种蛋白质，它们在紫外线照射、4-硝基喹啉-1-氧化物、X射线、百草枯和热休克的作用下，要么被诱导，要么电泳迁移率发生变化。发现有十种蛋白质对不止一种应激诱导剂有反应，这表明存在复杂的多肽诱导模式。本文讨论了这些发现的意义以及其中一些蛋白质对DNA修复过程和应激管理可能产生的影响。