Baker T I, Cords C E, Howard C A, Radloff R J
Department of Microbiology, School of Medicine, University of New Mexico, Albuquerque 87131.
Curr Genet. 1990 Oct;18(3):207-9. doi: 10.1007/BF00318382.
DNA repair mutants in eucaryotes are normally assigned to three epistasis groups. Each epistasis group represents a "pathway" for DNA repair. The pathways are commonly designated (1) nucleotide excision repair, (2) recombination repair and (3) mutagenic repair. An excision repair epistasis group has been established in Neurospora and the mutants assigned to this group should be limited in their ability to excise pyrimidine dimers and other bulky lesions from DNA. Using a pyrimidine dimer-specific assay, we have found that all Neurospora crassa mutants assigned to the excision repair epistasis group are capable of removing pyrimidine dimers from the DNA at a rate similar to the wild-type organism.
真核生物中的DNA修复突变体通常被分为三个上位性组。每个上位性组代表一种DNA修复“途径”。这些途径通常被指定为：(1) 核苷酸切除修复，(2) 重组修复，以及(3) 诱变修复。在粗糙脉孢菌中已经建立了一个切除修复上位性组，被分配到该组的突变体在从DNA中切除嘧啶二聚体和其他大分子损伤的能力上应该是有限的。使用一种嘧啶二聚体特异性检测方法，我们发现所有被分配到切除修复上位性组的粗糙脉孢菌突变体都能够以与野生型生物体相似的速率从DNA中去除嘧啶二聚体。
