Howard C A, Baker T I
Mol Gen Genet. 1986 Jun;203(3):462-7. doi: 10.1007/BF00422071.
The response of Neurospora crassa to DNA damage induced by UV irradiation has been studied using two-dimensional polyacrylamide gel electrophoresis (2-D PAGE). Whole cell extracts of irradiated and untreated cultures were compared. Five polypeptides that show changes in response to DNA damage have been identified. Several mutagen sensitive strains of Neurospora were also tested for polypeptide changes on 2-D PAGE. Profiles of whole cell extracts of these mutant strains were compared to wild type. Two changes were observed in the meiotic mutant, mei-3 and one change was detected in the excision repair mutant, upr-1. Two changes were also detected in the allelic mutants, uvs-3 and nuh-4. Profiles of uvs-3 and nuh-4 revealed one polypeptide that was missing and another polypeptide which appeared to shift to a more basic position. This same shift was detected in wild type after induction by UV irradiation or heat shock.
利用二维聚丙烯酰胺凝胶电泳（2-D PAGE）研究了粗糙脉孢菌对紫外线照射诱导的DNA损伤的反应。比较了辐照培养物和未处理培养物的全细胞提取物。已鉴定出五种对DNA损伤有反应变化的多肽。还对几种粗糙脉孢菌的诱变敏感菌株进行了二维聚丙烯酰胺凝胶电泳多肽变化测试。将这些突变菌株的全细胞提取物图谱与野生型进行了比较。在减数分裂突变体mei-3中观察到两个变化，在切除修复突变体upr-1中检测到一个变化。在等位基因突变体uvs-3和nuh-4中也检测到两个变化。uvs-3和nuh-4的图谱显示一种多肽缺失，另一种多肽似乎向更碱性的位置移动。在紫外线照射或热休克诱导后，野生型中也检测到了相同的迁移。
