Foss Simone R, Nakamura Celso V, Ueda-Nakamura Tania, Cortez Diógenes A G, Endo Eliana H, Dias Filho Benedito P
Post graduation in Microbiology, Universidade Estadual de Londrina, Londrina, PR, Brazil.
Department of Health Sciences, Universidade Estadual de Maringá, Maringá, PR, Brazil.
Ann Clin Microbiol Antimicrob. 2014 Sep 5;13:32. doi: 10.1186/s12941-014-0032-6.
Dermatophyte species infect the epidermis and appendages, often with serious social and health-economic consequences. The hydroalcoholic extract of pomegranate fruit peel showed activity against the dermatophyte fungi Trichophyton mentagrophytes, T. rubrum, Microsporum canis and M. gypseum.
Hydroalcoholic extract was prepared with pomegranate peels. This crude extract was fractionated and submitted to liquid-liquid partition, resulting in an active fraction which was fractionated in a Sephadex LH-20 column, followed by a Lobar column. The structure of the active compound was established with the use of spectroscopic methods.
The crude extract of pomegranate fruit peel showed activity against the dermatophytes Trichophyton mentagrophytes, T. rubrum, Microsporum canis, and M. gypseum, with MICs values of 125 μg/ml and 250 μg/ml, respectively for each genus. Punicalagin was isolated and identified by spectroscopic analysis. The crude extract and punicalagin showed activity against the conidial and hyphal stages of the fungi. The cytotoxicity assay showed selectivity for fungal cells than for mammalian cells.
These results indicated that the crude extract and punicalagin had a greater antifungal activity against T. rubrum, indicating that the pomegranate is a good target for study to obtain a new antidermatophyte medicine.
皮肤癣菌感染表皮及附属器，常造成严重的社会和健康经济后果。石榴果皮水醇提取物对皮肤癣菌须癣毛癣菌、红色毛癣菌、犬小孢子菌和石膏样小孢子菌具有活性。
用石榴皮制备水醇提取物。将该粗提物进行分馏并进行液-液分配，得到一种活性馏分，该活性馏分在葡聚糖凝胶LH-20柱上进行分馏，随后在Lobar柱上进行分馏。利用光谱方法确定活性化合物的结构。
石榴果皮粗提物对须癣毛癣菌、红色毛癣菌、犬小孢子菌和石膏样小孢子菌具有活性，各属的最低抑菌浓度分别为125μg/ml和250μg/ml。通过光谱分析分离并鉴定了安石榴苷。粗提物和安石榴苷对真菌的分生孢子和菌丝阶段均具有活性。细胞毒性试验表明，其对真菌细胞的选择性高于对哺乳动物细胞的选择性。
这些结果表明，粗提物和安石榴苷对红色毛癣菌具有较强的抗真菌活性，表明石榴是开发新型抗皮肤癣菌药物的良好研究对象。
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