一种Photinus pyralis（美洲萤火虫）和Luciola italica（意大利萤火虫）嵌合的萤火虫荧光素酶产生增强的生物发光。
A Photinus pyralis and Luciola italica chimeric firefly luciferase produces enhanced bioluminescence.
作者信息
Branchini Bruce R, Southworth Tara L, Fontaine Danielle M, Davis Audrey L, Behney Curran E, Murtiashaw Martha H
机构信息
Department of Chemistry, Connecticut College , New London, Connecticut 06320, United States.
出版信息
Biochemistry. 2014 Oct 14;53(40):6287-9. doi: 10.1021/bi501202u. Epub 2014 Oct 1.
We report the enhanced bioluminescence properties of a chimeric enzyme (PpyLit) that contains the N-domain of recombinant Photinus pyralis luciferase joined to the C-domain of recombinant Luciola italica luciferase. Compared to the P. pyralis enzyme, the novel PpyLit chimera exhibited 1.8-fold enhanced flash-height specific activity, 2.0-fold enhanced integration-based specific activity, 2.9-fold enhanced catalytic efficiency (kcat/Km), and a 1.4-fold greater bioluminescence quantum yield. The results of this study provide an underlying basis of this unusual example of a chimeric enzyme with enhanced catalytic properties that are not simply the sum of the contributions of the two luciferases.
我们报道了一种嵌合酶（PpyLit）增强的生物发光特性，该嵌合酶包含重组萤火虫荧光素酶的N结构域与重组意大利萤火虫荧光素酶的C结构域相连。与萤火虫荧光素酶相比，新型PpyLit嵌合体表现出1.8倍增强的闪光高度比活性、2.0倍增强的基于积分的比活性、2.9倍增强的催化效率（kcat/Km）以及1.4倍更高的生物发光量子产率。本研究结果为这种具有增强催化特性的嵌合酶这一不寻常例子提供了潜在基础，其催化特性并非简单地是两种荧光素酶贡献的总和。
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