Nishiyama Keita, Seto Yasuyuki, Yoshioka Kazuki, Kakuda Tsutomu, Takai Shinji, Yamamoto Yuji, Mukai Takao
Department of Animal Science, School of Veterinary Medicine, Kitasato University, Towada, Aomori, Japan.
Milk Science Research Institute, Megmilk Snow Brand Co. Ltd., Kawagoe, Saitama, Japan.
PLoS One. 2014 Sep 29;9(9):e108827. doi: 10.1371/journal.pone.0108827. eCollection 2014.
Campylobacter is a normal inhabitant of the chicken gut. Pathogenic infection with this organism in humans is accompanied by severe inflammation of the intestinal mucosal surface. The aim of this study was to evaluate the ability of Lactobacillus gasseri SBT2055 (LG2055) to inhibit the adhesion and invasion of Campylobacter jejuni in vitro and to suppress C. jejuni colonization of chicks in vivo. Pretreatment with LG2055 significantly reduced adhesion to and invasion of a human epithelial cell line, Intestine 407, by C. jejuni 81-176. Methanol (MeOH)-fixed LG2055 also reduced infection by C. jejuni 81-176. However, proteinase K (ProK)-treated LG2055 eliminated the inhibitory effects. Moreover, LG2055 co-aggregated with C. jejuni 81-176. ProK treatment prevented this co-aggregation, indicating that the co-aggregation phenotype mediated by the proteinaceous cell-surface components of LG2055 is important for reducing C. jejuni 81-176 adhesion and invasion. In an in vivo assay, oral doses of LG2055 were administered to chicks daily for 14 days after oral inoculation with C. jejuni 81-176. At 14 days post-inoculation, chicks treated with LG2055 had significantly reduced cecum colonization by C. jejuni. Reduction in the number of C. jejuni 81-176 cells adhering to and internalized by human epithelial cells demonstrated that LG2055 is an organism that effectively and competitively excludes C. jejuni 81-176. In addition, the results of the chick colonization assay suggest that treatment with LG2055 could be useful in suppressing C. jejuni colonization of the chicks at early growth stages.
弯曲杆菌是鸡肠道中的正常栖居菌。人类感染这种病原体后会伴有肠道黏膜表面的严重炎症。本研究的目的是评估加氏乳杆菌SBT2055（LG2055）在体外抑制空肠弯曲菌黏附和侵袭以及在体内抑制空肠弯曲菌在雏鸡体内定植的能力。用LG2055预处理可显著降低空肠弯曲菌81 - 176对人上皮细胞系Intestine 407的黏附和侵袭。甲醇（MeOH）固定的LG2055也可减少空肠弯曲菌81 - 176的感染。然而，蛋白酶K（ProK）处理的LG2055消除了抑制作用。此外，LG2055与空肠弯曲菌81 - 176发生共聚集。ProK处理可阻止这种共聚集，表明由LG2055的蛋白质性细胞表面成分介导的共聚集表型对于减少空肠弯曲菌81 - 176的黏附和侵袭很重要。在体内试验中，在给雏鸡口服接种空肠弯曲菌81 - 176后，每天给雏鸡口服LG2055，持续14天。接种后14天，用LG2055处理的雏鸡空肠弯曲菌在盲肠的定植显著减少。空肠弯曲菌81 - 176黏附并内化到人上皮细胞的数量减少表明LG2055是一种能有效且竞争性地排除空肠弯曲菌81 - 176的菌株。此外，雏鸡定植试验的结果表明，用LG2055处理可能有助于在雏鸡生长早期抑制空肠弯曲菌的定植。
