Ajami Abolghasem, Abedian Farshideh, Hamzeh Hosseini Seyyed, Akbarian Elahe, Alizadeh-Navaei Reza, Taghipour Mehrdad
Molecular and Cell-Biology Research Center, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran, e-mail:
Iran J Immunol. 2014 Sep;11(3):200-9.
Schizophrenia is a disorder of the executive function of both sensory and central nervous system. Recent studies suggest that immune mechanisms play a role in the pathophysiology of this disease. The variations in cytokine concentrations have been associated with psychopathology and treatment of schizophrenia.
To investigate the changes in serum concentrations of TNF-α, IL-10, and IL-2 in schizophrenic patients before and 40 days after treatment.
In a case-control study, 26 schizophrenic patients and 26 healthy individuals as a control group were enrolled. PANSS scale questionnaire was used for diagnosis and assessing the severity of the disease. All patients were then treated with risperidone or clozapine for 40 days. Serum concentrations of TNF-α, IL-10 and IL-2 were measured by ELISA before and after treatment in both groups. Paired t-test and Independent t-test were used for comparison of data.
Comparison of TNF-α and IL-10 concentrations in patients before and after treatment revealed a significance decrease of TNF-α and increase of IL-10 concentrations (p=0.002, and p=0.008, respectively). Serum concentrations of IL-2 were lower than the detection limit of assay and were not detectable. In comparison with healthy controls, serum concentrations of TNF-α in schizophrenic patients were higher, while IL-10 concentrations were lower before treatment although the differences were not significant (p=0.291 and p=0.375, respectively). There was no correlation between cytokine concentrations and the positive and negative scale (PANSS). Also no significant difference in the admission, relapses, and duration of illness before and after treatment was observed.
Increase of TNF-α and decrease of IL-10 may have an important role in psychopathology of schizophrenia.
精神分裂症是一种感觉和中枢神经系统执行功能的障碍性疾病。近期研究表明，免疫机制在该疾病的病理生理学中发挥作用。细胞因子浓度的变化与精神分裂症的精神病理学及治疗相关。
研究精神分裂症患者治疗前及治疗40天后血清中肿瘤坏死因子-α（TNF-α）、白细胞介素-10（IL-10）和白细胞介素-2（IL-2）浓度的变化。
在一项病例对照研究中，纳入26例精神分裂症患者及26名健康个体作为对照组。采用阳性和阴性症状量表（PANSS）问卷进行诊断及评估疾病严重程度。所有患者随后接受利培酮或氯氮平治疗40天。两组患者治疗前后均采用酶联免疫吸附测定（ELISA）法检测血清中TNF-α、IL-10和IL-2的浓度。采用配对t检验和独立t检验进行数据比较。
患者治疗前后TNF-α和IL-10浓度比较显示，TNF-α浓度显著降低，IL-10浓度升高（p值分别为0.002和0.008）。IL-2血清浓度低于检测限，无法检测到。与健康对照组相比，精神分裂症患者治疗前血清TNF-α浓度较高，而IL-10浓度较低，尽管差异无统计学意义（p值分别为0.291和0.375）。细胞因子浓度与阳性和阴性量表（PANSS）之间无相关性。治疗前后在入院率、复发率及病程方面也未观察到显著差异。
TNF-α升高和IL-10降低可能在精神分裂症的精神病理学中起重要作用。
