Coviello A, Soria M O, Proto M C, Berman D M, Gamundi S S, Alonso C E, de Bold A J
Department of Physiology, INSIBIO, School of Medicine, Universidad Nacional de Tucuman, Argentina.
Can J Physiol Pharmacol. 1989 Apr;67(4):362-5. doi: 10.1139/y89-058.
The atrial natriuretic peptide cardionatrin I (cardionatrin I is ANF 99-126) was used in studies directed to assess its effects on osmotic water permeability (Posm) and short-circuit current (SCC) in isolated toad skin. Results showed that ANF 99-126 (10(-7) M) added to the dermal side of the skin had no effect on basal Posm or SCC. However, ANF 99-126 (3.3 x 10(-8) M) was able to produce a 50% reversible inhibition of the maximal Posm response to angiotensin II (AII) (3.2 x 10(-8) M). These effects were seen when the skins were preincubated with ANF 99-126 for 10 min or less before the addition of AII. Longer preincubation appeared to inactivate ANF 99-126 through proteolysis. ANF 99-126(10(-7) M) failed to inhibit the SCC response to AII (10(-5) M) in toad skin. These results are compatible with a modulatory function for ANF on several systems including those involved in the regulation of extracellular fluid volume.
心房利钠肽心钠素I（心钠素I即ANF 99 - 126）被用于旨在评估其对离体蟾蜍皮肤渗透水通透性（Posm）和短路电流（SCC）影响的研究中。结果显示，添加到皮肤真皮侧的ANF 99 - 126（10⁻⁷M）对基础Posm或SCC没有影响。然而，ANF 99 - 126（3.3×10⁻⁸M）能够对血管紧张素II（AII，3.2×10⁻⁸M）引起的最大Posm反应产生50%的可逆性抑制。当在添加AII之前将皮肤与ANF 99 - 126预孵育10分钟或更短时间时，可观察到这些效应。更长时间的预孵育似乎通过蛋白水解作用使ANF 99 - 126失活。ANF 99 - 126（10⁻⁷M）未能抑制蟾蜍皮肤对AII（10⁻⁵M）的SCC反应。这些结果与ANF对包括参与细胞外液量调节的系统在内的多个系统具有调节功能相一致。
