Mitchell C L, McRee D I, Peterson N J, Tilson H A, Shandala M G, Rudnev M I, Varetskii V V, Navakatikyan M I
Laboratory of Molecular and Integrative Neuroscience, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709.
Environ Health Perspect. 1989 May;81:201-9. doi: 10.1289/ehp.8981201.
During the course of a formal program of cooperation between the United States and the Soviet Union concerning the biological effects of physical factors in the environment, it was concluded that duplicate projects should be initiated with the general goal of determining the most sensitive and valid test procedures for evaluating the effects of microwave radiation on the central nervous system. This report details an initial step in this direction. Male rats of the Fischer 344 strain were exposed or sham exposed to 10 mW/cm2 continuous wave microwave radiation at 2.45 GHz for a period of 7 hr. Animals were subjected to behavioral, biochemical, or electrophysiological measurements during and/or immediately after exposure. Behavioral tests used were passive avoidance and activity in an open field. Biochemical measurements were ATPase (Na+, K+; Mg2+, Ca2+) and K+ alkaline phosphatase activities. Electrophysiological measurements consisted of EEG frequency analysis. Neither group observed a significant effect of microwave irradiation on open field activity. Both groups observed changes in variability of the data obtained using the passive avoidance procedure, but not in the same parameters. The U.S. group, but not the USSR group, found significantly less Na+,K+-ATPase activity in the microwave-exposed animals compared to the sham exposed animals. Both groups found incidences of statistically significant effects in the power spectral analysis of EEG frequency, but not at the same frequency. The failure of both groups to substantiate the results of the other reinforces our contention that such duplicate projects are important and necessary.
在美国与苏联就环境中物理因素的生物学效应开展正式合作项目的过程中，得出的结论是，应启动重复项目，总体目标是确定评估微波辐射对中枢神经系统影响的最敏感且有效的测试程序。本报告详细介绍了朝这个方向迈出的第一步。将Fischer 344品系的雄性大鼠暴露于2.45 GHz、10 mW/cm²的连续波微波辐射下7小时，或进行假暴露。在暴露期间和/或暴露后立即对动物进行行为、生化或电生理测量。所使用的行为测试是被动回避和旷场活动。生化测量是ATP酶（Na⁺，K⁺；Mg²⁺，Ca²⁺）和K⁺碱性磷酸酶活性。电生理测量包括脑电图频率分析。两组均未观察到微波辐射对旷场活动有显著影响。两组均观察到使用被动回避程序获得的数据变异性有变化，但不是相同的参数。与假暴露动物相比，美国组（而非苏联组）发现微波暴露动物的Na⁺，K⁺ - ATP酶活性显著降低。两组在脑电图频率的功率谱分析中均发现有统计学显著效应的发生率，但不是在相同频率。两组均未能证实对方的结果，这强化了我们的观点，即此类重复项目很重要且必要。
