Yamazaki Toyomi, Sagisaka Miki, Ikeda Riko, Nakamura Toshiyuki, Matsuda Noriko, Ishii Takeshi, Nakayama Tsutomu, Watanabe Tatsuo
a Graduate School of Integrated Pharmaceutical and Nutritional Sciences, University of Shizuoka , Shizuoka , Japan.
Biosci Biotechnol Biochem. 2014;78(10):1753-6. doi: 10.1080/09168451.2014.930326. Epub 2014 Jun 26.
We purified several hundred mgs of four major theaflavins (theaflavin, theaflavin-3-O-gallate, theaflavin-3'-O-gallate, and theaflavin-3,3'-O-digallate). Among the 25 hTAS2Rs expressed in HEK293T cells, hTAS2R39 and hTAS2R14 were activated by theaflavins. Both hTAS2R39 and hTAS2R14 responded to theaflavin-3'-O-gallate. In addition, hTAS2R39 was activated by theaflavin and theaflavin-3,3'-O-gallate, but not by theaflavin-3-O-gallate. In contrast, hTAS2R14 responded to theaflavin-3-O-gallate.
我们纯化了几百毫克的四种主要茶黄素（茶黄素、茶黄素-3-O-没食子酸酯、茶黄素-3'-O-没食子酸酯和茶黄素-3,3'-O-双没食子酸酯）。在HEK293T细胞中表达的25种人味觉感受器2型受体（hTAS2Rs）中，hTAS2R39和hTAS2R14被茶黄素激活。hTAS2R39和hTAS2R14都对茶黄素-3'-O-没食子酸酯有反应。此外，hTAS2R39被茶黄素和茶黄素-3,3'-O-双没食子酸酯激活，但不被茶黄素-3-O-没食子酸酯激活。相反，hTAS2R14对茶黄素-3-O-没食子酸酯有反应。
