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纤连蛋白受体在CHO细胞中的内吞作用与再循环

Endocytosis and recycling of the fibronectin receptor in CHO cells.

作者信息

Bretscher M S

机构信息

Medical Research Council, Laboratory of Molecular Biology, Cambridge, UK.

出版信息

EMBO J. 1989 May;8(5):1341-8. doi: 10.1002/j.1460-2075.1989.tb03514.x.

DOI:10.1002/j.1460-2075.1989.tb03514.x
PMID:2527741
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC400960/
Abstract

An anti-fibronectin receptor monoclonal antibody preferentially labels the leading edges of freshly plated CHO fibroblasts, suggesting that this receptor circulates through the endocytic cycle. Using a new labelling reagent, I show that this receptor is indeed endocytosed at 37 degrees C and then returned to the cell surface. These findings imply that fibronectin receptors are recirculated to the leading edge of a motile cell by the endocytic cycle, and establish that the processes of endocytosis/exocytosis and cell locomotion are intimately linked.

摘要

一种抗纤连蛋白受体单克隆抗体优先标记新接种的中国仓鼠卵巢（CHO）成纤维细胞的前缘，这表明该受体通过内吞循环进行循环。使用一种新的标记试剂，我发现该受体在37摄氏度时确实被内吞，然后又回到细胞表面。这些发现意味着纤连蛋白受体通过内吞循环再循环到运动细胞的前缘，并证实内吞/外排过程与细胞运动密切相关。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f669/400960/9c03c4cc81d6/emboj00129-0061-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f669/400960/cdd0f03c54ca/emboj00129-0058-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f669/400960/45b036ee61e7/emboj00129-0059-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f669/400960/456df466fac2/emboj00129-0060-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f669/400960/a7c8d3725e17/emboj00129-0060-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f669/400960/9c03c4cc81d6/emboj00129-0061-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f669/400960/cdd0f03c54ca/emboj00129-0058-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f669/400960/45b036ee61e7/emboj00129-0059-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f669/400960/456df466fac2/emboj00129-0060-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f669/400960/a7c8d3725e17/emboj00129-0060-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f669/400960/9c03c4cc81d6/emboj00129-0061-a.jpg

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