Núñez Ivanna Novotny, Galdeano Carolina Maldonado, de LeBlanc Alejandra de Moreno, Perdigón Gabriela
Centro de Referencia para Lactobacilos (CERELA-CONICET), San Miguel de Tucumán, Tucumán, Argentina.
Centro de Referencia para Lactobacilos (CERELA-CONICET), San Miguel de Tucumán, Tucumán, Argentina; Cátedra de Inmunología. Instituto de Microbiología, Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán, Tucumán, Argentina.
Nutrition. 2014 Nov-Dec;30(11-12):1423-32. doi: 10.1016/j.nut.2014.03.025. Epub 2014 Apr 13.
Obesity is associated with alterations in intestinal microbiota and immunity. The aim of this study was to determine the effect of probiotic Lactobacillus casei CRL 431 administration on intestinal and humoral immune response, clinical parameters, and gut microbiota was evaluated using a high-fat diet to induce obesity in a mouse model.
Adult mice received a conventional balanced diet or a high-fat diet supplemented with milk, milk fermented by Lactobacillus casei (FM), L. casei as suspension, or water over 60 d. Histology of liver and small intestine (SI), immunoglobulin A-positive cells and macrophages in SI, phagocytic activity of spleen and peritoneal macrophages, and humoral immune response to ovalbumin were studied. Clinical parameters in serum and gut microbiota were also analyzed.
FM was the most effective supplement for decreasing body weight and clinical parameters in serum. The histology of liver and SI was also improved in obese mice given FM. These animals had increased numbers of immunoglobulin A-positive cells and macrophages in SI. The gut microbiota showed that obese mice given probiotics had increased Bacteroides and bifidobacteria. Administration of FM or L. casei as suspension enhanced the phagocytic activity of macrophages. The anti-ovalbumin specific immune response was not increased by any supplement assayed.
Administration of probiotics to obese hosts improved the gut microbiota and the mucosal immunity altered by obesity, down-regulated some biochemical parameters in blood associated with metabolic syndrome, and decreased liver steatosis. These results demonstrate the potential use of probiotics in obese individuals to decrease the body weight and to improve the biochemical and immunologic parameters altered by obesity.
肥胖与肠道微生物群和免疫力的改变有关。本研究旨在确定给予益生菌干酪乳杆菌CRL 431对肠道和体液免疫反应的影响，使用高脂饮食诱导小鼠模型肥胖，评估临床参数和肠道微生物群。
成年小鼠在60天内接受常规平衡饮食或补充牛奶、干酪乳杆菌发酵牛奶（FM）、干酪乳杆菌悬浮液或水的高脂饮食。研究肝脏和小肠（SI）的组织学、SI中的免疫球蛋白A阳性细胞和巨噬细胞、脾脏和腹膜巨噬细胞的吞噬活性以及对卵清蛋白的体液免疫反应。还分析了血清中的临床参数和肠道微生物群。
FM是降低体重和血清临床参数最有效的补充剂。给予FM的肥胖小鼠肝脏和SI的组织学也得到改善。这些动物SI中的免疫球蛋白A阳性细胞和巨噬细胞数量增加。肠道微生物群显示，给予益生菌的肥胖小鼠中拟杆菌和双歧杆菌增加。给予FM或干酪乳杆菌悬浮液可增强巨噬细胞的吞噬活性。任何检测的补充剂均未增加抗卵清蛋白特异性免疫反应。
给肥胖宿主施用益生菌可改善肠道微生物群和因肥胖而改变的粘膜免疫，下调与代谢综合征相关的血液中的一些生化参数，并减少肝脏脂肪变性。这些结果证明了益生菌在肥胖个体中用于减轻体重和改善因肥胖而改变的生化和免疫参数的潜在用途。
