Lalitha R, George R, Ramasarma T
Department of Biochemistry, Indian Institute of Science, Bangalore.
Mol Cell Biochem. 1989 Jun 1;87(2):161-70. doi: 10.1007/BF00219259.
The activities of the mevalonate metabolizing enzymes-HMG-CoA reductase, mevalonate kinase, mevalonate phosphokinase and mevalonate pyrophosphate decarboxylase -were assayed with the respective substrates in green seedlings of Arachis hypogaea. MVAPP decarboxylase is the rate-limiting step among these enzymes and is inhibited by phenolic acids. Its activity in the seedlings was found to decrease in the absence of light and on treatment with abscisic acid. These results suggest that regulation of isoprene pathway in groundnut seedlings may occur at the level of mevalonate decarboxylation.
在花生绿色幼苗中，使用相应底物测定了甲羟戊酸代谢酶——HMG-CoA还原酶、甲羟戊酸激酶、甲羟戊酸磷酸激酶和甲羟戊酸焦磷酸脱羧酶的活性。甲羟戊酸焦磷酸脱羧酶是这些酶中的限速步骤，并且受到酚酸的抑制。发现在无光照和用脱落酸处理时，幼苗中该酶的活性会降低。这些结果表明，花生幼苗中异戊二烯途径的调控可能发生在甲羟戊酸脱羧水平。
