Sahiner Nurettin
Faculty of Science & Arts, Chemistry Department, Canakkale Onsekiz Mart University, Terzioglu Campus, 17100 Canakkale, Turkey; Nanoscience and Technology Research and Application Center (NANORAC), Canakkale Onsekiz Mart University, Terzioglu Campus, 17100 Canakkale, Turkey.
Mater Sci Eng C Mater Biol Appl. 2014 Nov;44:9-16. doi: 10.1016/j.msec.2014.08.009. Epub 2014 Aug 7.
Poly(rutin) p(RT) particles were prepared for the first time via a simple microemulsion polymerization/crosslinking method using l-α lecithin as surfactant, cyclohexane as organic phase and glycerol diglycidyl ether (GDE) as a crosslinking agent. Highly negatively charged p(RT) particles, -48.2 mV, were obtained due to phenolic groups on the particles. It was also confirmed that p(RT) particles are thermally more stable than RT and degradable in PBS at pH7.4., e.g., 11 wt.% can degrade in 1 day and little further degradation was observed over 9 days. The prepared p(RT) particles showed insignificant antibacterial characteristics against common bacteria such as Escherichia coli ATCC8739, Staphylococcus aureus ATCC6538, and Bacillus subtilis ATCC6633 whereas the RT molecules showed significantly better antibacterial characteristics even at low concentrations. Moreover, p(RT) particles were demonstrated for use as drug delivery devices by loading rosmarinic acid (RA) as model drug and showed release capability for up to 6 days by releasing 85% of the loaded RA. Intriguingly, p(RT) particles illustrated enhanced fluorescent properties providing great potential for fluorescent active antioxidant and antibacterial materials in biomedical use.
首次通过一种简单的微乳液聚合/交联方法制备了聚（芦丁）p（RT）颗粒，该方法使用l-α卵磷脂作为表面活性剂，环己烷作为有机相，甘油二缩水甘油醚（GDE）作为交联剂。由于颗粒上的酚基，获得了高带负电荷的p（RT）颗粒，其ζ电位为-48.2 mV。还证实了p（RT）颗粒在热稳定性上比芦丁（RT）更高，并且在pH7.4的磷酸盐缓冲盐溶液（PBS）中可降解，例如，11 wt.% 在1天内可降解，在9天内几乎没有进一步降解。制备的p（RT）颗粒对常见细菌如大肠杆菌ATCC8739、金黄色葡萄球菌ATCC6538和枯草芽孢杆菌ATCC6633显示出微不足道的抗菌特性，而RT分子即使在低浓度下也显示出明显更好的抗菌特性。此外，通过负载迷迭香酸（RA）作为模型药物，证明了p（RT）颗粒可用作药物递送装置，并通过释放85% 的负载RA显示出长达6天的释放能力。有趣的是，p（RT）颗粒表现出增强的荧光特性，为生物医学用途中的荧光活性抗氧化剂和抗菌材料提供了巨大潜力。
