嗜热链状地芽孢杆菌GS-1菌株的永久基因组草图序列

Permanent draft genome sequence of Geobacillus thermocatenulatus strain GS-1.

作者信息

Zheng Beiwen, Zhang Fan, Chai Lujun, Yu Gaoming, Shu Fuchang, Wang Zhengliang, Su Sanbao, Xiang Tingsheng, Zhang Zhongzhi, Hou DuJie, She Yuehui

机构信息

State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, Zhejiang University, Hangzhou, China.

The Key Laboratory of Marine Reservoir Evolution and Hydrocarbon Accumulation Mechanism, School of Energy Resources, China University of Geosciences, Beijing, China.

出版信息

Mar Genomics. 2014 Dec;18PB:129-131. doi: 10.1016/j.margen.2014.09.005. Epub 2014 Oct 1.

DOI:10.1016/j.margen.2014.09.005

PMID:25280889

Abstract

Geobacillus thermocatenulatus strain GS-1 is a thermophilic bacillus having a growth optimum at 60°C, capable of degrading alkanes. It was isolated from the formation water of a high-temperature deep oil reservoir in Qinghai oilfield, China. Here, we report the draft genome sequence with an estimated assembly size of 3.5Mb. A total of 3371 protein-coding sequences, including monooxygenase, alcohol dehydrogenase, aldehyde dehydrogenase, fatty acid-CoA ligase, acyl-CoA dehydrogenase, enoyl-CoA hydrogenase, hydroxyacyl-CoA dehydrogenase and thiolase, were detected in the genome, which are involved in the alkane degradation pathway. Our results may provide insights into the genetic basis of the adaptation of this strain to high-temperature oilfield ecosystems.

摘要

嗜热串联芽孢杆菌GS-1菌株是一种嗜热芽孢杆菌，最适生长温度为60°C，能够降解烷烃。它是从中国青海油田高温深层油藏的地层水中分离出来的。在此，我们报告了其基因组草图序列，估计组装大小为3.5Mb。在基因组中总共检测到3371个蛋白质编码序列，包括单加氧酶、醇脱氢酶、醛脱氢酶、脂肪酸辅酶A连接酶、酰基辅酶A脱氢酶、烯酰辅酶A氢化酶、羟酰辅酶A脱氢酶和硫解酶，它们参与烷烃降解途径。我们的结果可能为该菌株适应高温油田生态系统的遗传基础提供见解。