β-环糊精包覆的 CdSe/ZnS 量子点用于食品样品中香草醛的传感。

β-Cyclodextrin coated CdSe/ZnS quantum dots for vanillin sensoring in food samples.

机构信息

Department of Analytical Chemistry and Food Technology, University of Castilla-La Mancha, Ciudad Real, Spain; IRICA (Regional Institute of Applied Scientific Research). Avenida Camilo José Cela, s/n. 13071 Ciudad Real, Spain.

Department of Analytical Chemistry and Food Technology, University of Castilla-La Mancha, Ciudad Real, Spain.

出版信息

Talanta. 2015 Jan;131:286-91. doi: 10.1016/j.talanta.2014.07.100. Epub 2014 Aug 8.

DOI:10.1016/j.talanta.2014.07.100

PMID:25281104

Abstract

An optical sensor for vanillin in food samples using CdSe/ZnS quantum dots (QDs) modified with β-cyclodextrin (β-CD) was developed. This vanillin-sensor is based on the selective host-guest interaction between vanillin and β-cyclodextrin. The procedure for the synthesis of β-cyclodextrin-CdSe/ZnS (β-CD-CdSe/ZnS-QDs) complex was optimized, and its fluorescent characteristics are reported. It was found that the interaction between vanillin and β-CD-CdSe/ZnS-QDs complex produced the quenching of the original fluorescence of β-CD-CdSe/ZnS-QDs according to the Stern-Volmer equation. The mechanism of the interaction is discussed. The analytical potential of this sensoring system was demonstrated by the determination of vanillin in synthetic and food samples. The method was selective for vanillin, with a limit of detection of 0.99 µg mL(-1), and a reproducibility of 4.1% in terms of relative standard deviation (1.2% under repeatability conditions). Recovery values were in the 90-105% range for food samples.

摘要

采用β-环糊精（β-CD）修饰的 CdSe/ZnS 量子点（QDs）开发了一种用于食品样品中香草醛的光学传感器。这种香草醛传感器基于香草醛和β-环糊精之间的选择性主客体相互作用。优化了β-环糊精-CdSe/ZnS（β-CD-CdSe/ZnS-QDs）复合物的合成工艺，并报告了其荧光特性。结果发现，根据 Stern-Volmer 方程，香草醛与β-CD-CdSe/ZnS-QDs 复合物之间的相互作用导致了β-CD-CdSe/ZnS-QDs 原始荧光的猝灭。讨论了相互作用的机制。通过对合成和食品样品中香草醛的测定，证明了这种传感系统的分析潜力。该方法对香草醛具有选择性，检测限为 0.99µg mL(-1)，相对标准偏差的重现性为 4.1%（重复性条件下为 1.2%）。对于食品样品，回收率值在 90-105%范围内。