Sirota L, Kupfer B, Moroz C
Neonatal Unit, Hasharon Hospital, Tel Aviv, Israel.
Clin Exp Immunol. 1989 Aug;77(2):257-62.
CM-H-9 monoclonal antibody specific for placental isoferritin (PLF) was used to quantify PLF in the serum and on peripheral blood lymphocytes derived from term delivery women and healthy controls. It was found that the mean level of PLF in maternal sera was 50.4 +/- 50.1 U/ml, whereas in normal adults the mean serum PLF level was very low (4.5 +/- 7.7 U/ml). Furthermore, term mothers exhibited a sub-population of peripheral blood lymphocytes (PBL) which stained positively with CM-H-9 MoAb (mean 11.6 +/- 7.8%). Such lymphocytes were scarce in normal non-pregnant women (mean 0.8 +/- 1.2%). The effect of PLF on lymphocyte transformation in MLC was studied in mixed lymphocyte cultures of maternal-newborn and normal non-related PBL controls. It was found that placental isoferritin was immunosuppressive in comparison to normal adult ferritin. The suppressive effect was significantly higher in maternal-newborn MLC compared to normal adult controls. Furthermore, it was found that PLF which is present in maternal serum has an immunosuppressive activity since, its removal on CM-H-9 MoAb affinity column abrogated this effect. The results of this study suggest that both PLF and PLF binding lymphocytes play a role in the development of immunosuppression during pregnancy. Therefore the lack of one of the factors may result in diminished immunosuppression or in fetal rejection.
使用针对胎盘铁蛋白（PLF）的CM-H-9单克隆抗体对足月分娩妇女和健康对照者外周血淋巴细胞及血清中的PLF进行定量。结果发现，孕妇血清中PLF的平均水平为50.4±50.1 U/ml，而正常成年人血清PLF平均水平很低（4.5±7.7 U/ml）。此外，足月产妇外周血淋巴细胞（PBL）中有一个亚群能被CM-H-9单克隆抗体阳性染色（平均为11.6±7.8%）。而这种淋巴细胞在正常未孕妇女中很少见（平均为0.8±1.2%）。在母婴和正常非亲属PBL对照的混合淋巴细胞培养中研究了PLF对MLC中淋巴细胞转化的影响。结果发现，与正常成人铁蛋白相比，胎盘铁蛋白具有免疫抑制作用。与正常成人对照相比，母婴MLC中的抑制作用明显更高。此外，还发现孕妇血清中的PLF具有免疫抑制活性，因为其在CM-H-9单克隆抗体亲和柱上被去除后这种作用就消失了。本研究结果表明，PLF和与PLF结合的淋巴细胞在孕期免疫抑制的发生过程中均发挥作用。因此，其中一个因素的缺失可能导致免疫抑制减弱或胎儿排斥反应。
