Vatsalya Vatsalya, Arora Kashmiri L
Department of Veterinary Science, Fort Valley State University, Fort Valley, Georgia, USA.
Int J Poult Sci. 2011 Sep;10(9):680-684. doi: 10.3923/ijps.2011.680.684.
Japanese quail is very popular research animal model. Its continued characterization for various norms is highly desirable for obtaining accurate and reliable results. This study was designed to assess various physiological parameters which are associated with body growth and development. Among various physiological parameters, blood constituents and hormones are commonly used as diagnostic tools in both physiological and pathological evaluations of humans and animals. Japanese quail hatchlings were housed in the temperature controlled brooders up to 3 weeks of age and then shifted to hanging cages in air conditioned room at ~74 F under 14L:10D lighting system and free access to feed and fresh water. Starting d8, a group of birds of uniform size and weight were selected randomly and euthanized at 4-day intervals up to d52 of age. The birds were weighed and blood sampled from the brachial vein for measuring Blood Glucose (BGL), Total Plasma Proteins (PP) and Packed Cell Volume (PCV). It was found that starting d36 all the three physiological parameters altered with approaching sexual maturity (d48-52): BGL decreased (252 vrs. 182 mg/dl, p<0.05), PCV% increased (43.6 vrs. 49.6%, p<0.05) and PP also increased (2.7 vrs. 3.2 gm/dl, p>0.05). Accordingly, BGL, PCV and PP values demonstrated significant potential to predict approaching sexual maturity in male Japanese quail.
日本鹌鹑是一种非常受欢迎的研究动物模型。为了获得准确可靠的结果,非常有必要对其各种标准进行持续的特征描述。本研究旨在评估与身体生长发育相关的各种生理参数。在各种生理参数中,血液成分和激素在人和动物的生理及病理评估中通常被用作诊断工具。日本鹌鹑幼雏在温度可控的育雏器中饲养至3周龄,然后转移到空调房间的悬挂笼中,温度约为74华氏度,光照系统为14小时光照:10小时黑暗,自由采食和饮用新鲜水。从第8天开始,随机选择一组大小和体重一致的鸟,每隔4天进行安乐死,直至52日龄。对这些鸟进行称重,并从肱静脉采集血液样本,以测量血糖(BGL)、总血浆蛋白(PP)和红细胞压积(PCV)。结果发现,从第36天开始,随着性成熟临近(48 - 52日龄),所有这三个生理参数都发生了变化:血糖降低(252对182毫克/分升,p<0.05),红细胞压积百分比增加(43.6对49.6%,p<0.05),总血浆蛋白也增加(2.7对3.2克/分升,p>0.05)。因此,血糖、红细胞压积和总血浆蛋白值显示出显著的潜力来预测雄性日本鹌鹑性成熟的临近。