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水动力在脂肪移植中会产生影响吗?关于提高脂肪抽吸物活力和脂肪移植存活率的体外和体内证据。

Does water-jet force make a difference in fat grafting? In vitro and in vivo evidence of improved lipoaspirate viability and fat graft survival.

作者信息

Yin Shilu, Luan Jie, Fu Su, Wang Qian, Zhuang Qiang

机构信息

Beijing, People's Republic of China From the Breast Plastic and Reconstructive Surgery Center and the Department of Pathology, Plastic Surgery Hospital, and the Plastic Surgery Institute, Chinese Academy of Medical Sciences, Peking Union Medical College.

出版信息

Plast Reconstr Surg. 2015 Jan;135(1):127-138. doi: 10.1097/PRS.0000000000000780.

DOI:10.1097/PRS.0000000000000780
PMID:25285679
Abstract

BACKGROUND

Recent literature has revealed that water-jet-assisted liposuction offers a new method of conventional liposuction techniques by using the gentle spray of fluid. However, there has not yet been a systematic, randomized, controlled study to demonstrate its effect on the vitality and postoperative fat survival of fresh lipoaspirates. In this study, the authors compared liposuction with or without water-jet assistance in a blinded fashion.

METHODS

Human lipoaspirates were obtained from healthy Chinese female volunteers for body shaping. Lipoaspirates were harvested by a single surgeon using the same material and machine; water-jet assistance was the only variance in this study. At the beginning of surgery, the authors randomly performed conventional manual liposuction without water-jet assistance for one side to obtain 50 ml of lipoaspirate (group B). At the corresponding area of the other side, the authors used water-jet-assisted liposuction to obtain another 50 ml of lipoaspirate (group A). All of the harvested lipoaspirates were used in the in vitro and in vivo experiments to evaluate the effect of water-jet force on the vitality and postoperative fat survival of fresh lipoaspirates.

RESULTS

Fresh lipoaspirates from group A had greater viability and a higher percentage of CD34/CD45 cells than group B. Grafted lipoaspirates in group A had better weight retention, less apoptosis, and greater angiogenesis.

CONCLUSIONS

The fate of grafted lipoaspirates was affected by water-jet force. With the assistance of water-jet force during the harvesting procedure, the authors could obtain more viable lipoaspirates and achieve better fat survival.

摘要

背景

近期文献表明,水刀辅助吸脂术通过温和的液体喷射提供了一种传统吸脂技术的新方法。然而,尚未有系统的、随机对照研究来证明其对新鲜脂肪抽吸物活力和术后脂肪存活率的影响。在本研究中,作者以盲法比较了有水刀辅助和无水刀辅助的吸脂术。

方法

从健康中国女性志愿者身上获取人体脂肪抽吸物用于身体塑形。脂肪抽吸物由一名外科医生使用相同材料和机器采集;本研究中唯一的变量是水刀辅助。手术开始时,作者随机在一侧进行无水刀辅助的传统手动吸脂术以获取50毫升脂肪抽吸物(B组)。在另一侧的相应区域,作者使用水刀辅助吸脂术获取另外50毫升脂肪抽吸物(A组)。所有采集的脂肪抽吸物均用于体外和体内实验,以评估水刀力量对新鲜脂肪抽吸物活力和术后脂肪存活率的影响。

结果

A组新鲜脂肪抽吸物的活力更高,CD34/CD45细胞百分比高于B组。A组移植的脂肪抽吸物重量保留更好,凋亡更少,血管生成更多。

结论

移植脂肪抽吸物的命运受水刀力量影响。在采集过程中借助水刀力量,作者可以获得更有活力的脂肪抽吸物并实现更好的脂肪存活。

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