Taha Sara, Saller Maximilian Michael, Haas Elisabeth, Farkas Zsuzsanna, Aszodi Attila, Giunta Riccardo, Volkmer Elias
Department of Hand, Plastic and Aesthetic Surgery, Ludwig-Maximilians-University (LMU), Pettenkoferstraße. 8a, 80336 Munich, Germany.
Experimental Surgery and Regenerative Medicine (ExperiMed), Department of General, Trauma and Reconstructive Surgery, Ludwig-Maximilians-University (LMU), Nußbaumstraße 20, 80336 Munich, Germany.
J Plast Reconstr Aesthet Surg. 2020 Jan;73(1):166-175. doi: 10.1016/j.bjps.2019.06.025. Epub 2019 Jun 28.
Adipose-derived stem/progenitor cells (ADSPCs) are under investigation in many clinical applications for their regenerative potential in a variety of autoimmune, degenerative, and inflammatory diseases. Adipose tissue, which is mainly harvested by manual liposuction, is the main source of these ADSPCs.
In the past years, a variety of different liposuction devices have been commercialized. To ensure a high quality of obtained ADSPCs, it is crucial to show the advantages and disadvantages of frequently used liposuction methods. For this reason, the objective of this study was to compare ADSPCs harvested by either the suction-assisted LipiVage200-5 or the water-assisted Body-Jet system.
The proliferation potential of ADSPCs, harvested from 20 patients, was assessed by cumulative population doublings (cumPD), population doubling time (PDT), colony-forming units (CFU), and cell metabolism assays. To prove the multipotency of the primary isolated cells, ADSPCs were induced to differentiate into adipogenic, osteogenic, and chondrogenic lineages.
Our data show a significantly higher cumPD, as well as a significantly lower PDT for cells obtained by the Body-Jet system. No significant differences were found regarding the CFU efficiency and the cell metabolism. Furthermore, we showed that the adipogenic, osteogenic, and chondrogenic potential of ADSPCs is similar in both groups.
DISCUSSION/CONCLUSION: In our study, we provide evidence that the cell characteristics and the functional properties of ADSPCs isolated after liposuction with different techniques are largely similar. However, we observed a significantly higher cumPD and a slightly higher adipogenic potential in cells isolated after liposuction with the Body-Jet system. Different cannula sizes and sheer stresses in the used methods might play a role here.
脂肪来源的干/祖细胞（ADSPCs）因其在多种自身免疫性、退行性和炎性疾病中的再生潜力而正被用于许多临床应用研究。脂肪组织主要通过手动抽脂获取，是这些ADSPCs的主要来源。
在过去几年中，多种不同的抽脂设备已商业化。为确保所获得的ADSPCs的高质量，阐明常用抽脂方法的优缺点至关重要。因此，本研究的目的是比较通过吸脂辅助LipiVage200 - 5或水辅助Body - Jet系统获取的ADSPCs。
通过累积群体倍增数（cumPD）、群体倍增时间（PDT）、集落形成单位（CFU）和细胞代谢测定，评估从20例患者获取的ADSPCs的增殖潜力。为证明原代分离细胞的多能性，诱导ADSPCs分化为脂肪生成、骨生成和软骨生成谱系。
我们的数据显示，通过Body - Jet系统获得的细胞具有显著更高的cumPD以及显著更低的PDT。在CFU效率和细胞代谢方面未发现显著差异。此外，我们表明两组中ADSPCs的脂肪生成、骨生成和软骨生成潜力相似。
讨论/结论：在我们的研究中，我们提供证据表明，用不同技术抽脂后分离的ADSPCs的细胞特征和功能特性在很大程度上相似。然而，我们观察到用Body - Jet系统抽脂后分离的细胞具有显著更高的cumPD和略高的脂肪生成潜力。所用方法中不同的套管尺寸和剪切应力可能在此起作用。
