Nilsson Bradley L, Soellner Matthew B, Raines Ronald T
Department of Chemistry, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA.
Annu Rev Biophys Biomol Struct. 2005;34:91-118. doi: 10.1146/annurev.biophys.34.040204.144700.
Proteins have become accessible targets for chemical synthesis. The basic strategy is to use native chemical ligation, Staudinger ligation, or other orthogonal chemical reactions to couple synthetic peptides. The ligation reactions are compatible with a variety of solvents and proceed in solution or on a solid support. Chemical synthesis enables a level of control on protein composition that greatly exceeds that attainable with ribosome-mediated biosynthesis. Accordingly, the chemical synthesis of proteins is providing previously unattainable insight into the structure and function of proteins.
蛋白质已成为化学合成可及的目标。基本策略是使用天然化学连接、施陶丁格连接或其他正交化学反应来偶联合成肽段。这些连接反应与多种溶剂兼容，可在溶液中或在固体支持物上进行。化学合成能够对蛋白质组成进行控制，其程度大大超过核糖体介导的生物合成所能达到的水平。因此，蛋白质的化学合成正在为蛋白质的结构和功能提供前所未有的深入见解。
