Walsh L J, Kaminer M S, Lazarus G S, Lavker R M, Murphy G F
Department of Dermatology, University of Pennsylvania School of Medicine, Philadelphia.
Lab Invest. 1991 Oct;65(4):433-40.
Human connective tissue type mast cells (CTMC) are frequently located in close proximity to microvascular and neural basement membranes (BM). We have explored the interaction between human dermal connective tissue-type (chymase positive) mast cells and laminin, a component of BM. In this report, we document that normal CTMC express laminin receptors and are intimately associated with laminin of BM in vivo and pericellular laminin complexes in vitro. Upon degranulation in vitro, CTMC-laminin complexes dissociate and CTMC do not adhere to laminin substrates. In cutaneous mastocytosis/urticaria pigmentosa, CTMC do not express laminin receptors detectable by immunohistochemistry, and are frequently not in close association with laminin of vascular BM. These same features could be induced by degranulation of normal mast cells in organ culture. These findings indicate that CTMC-laminin interactions may be important determinants of mast cell localization in tissue compartments.
人类结缔组织型肥大细胞（CTMC）常紧邻微血管和神经基底膜（BM）。我们研究了人类真皮结缔组织型（糜蛋白酶阳性）肥大细胞与基底膜成分层粘连蛋白之间的相互作用。在本报告中，我们证明正常CTMC表达层粘连蛋白受体，在体内与基底膜层粘连蛋白紧密相关，在体外与细胞周围层粘连蛋白复合物紧密相关。体外脱颗粒时，CTMC-层粘连蛋白复合物解离，CTMC不再黏附于层粘连蛋白底物。在皮肤肥大细胞增多症/色素性荨麻疹中，免疫组化检测不到CTMC表达层粘连蛋白受体，且常与血管基底膜层粘连蛋白无紧密关联。在器官培养中，正常肥大细胞脱颗粒也可诱导出这些相同特征。这些发现表明，CTMC-层粘连蛋白相互作用可能是肥大细胞在组织区室中定位的重要决定因素。
