Lee Min Hee, Kim Jong Seung, Sessler Jonathan L
Department of Chemistry, The University of Texas at Austin, Austin, Texas 78712-1224, USA.
Chem Soc Rev. 2015 Jul 7;44(13):4185-91. doi: 10.1039/c4cs00280f. Epub 2014 Oct 6.
Quantitative determination of specific analytes is essential for a variety of applications ranging from life sciences to environmental monitoring. Optical sensing allows non-invasive measurements within biological milieus, parallel monitoring of multiple samples, and less invasive imaging. Among the optical sensing methods currently being explored, ratiometric fluorescence sensing has received particular attention as a technique with the potential to provide precise and quantitative analyses. Among its advantages are high sensitivity and inherent reliability, which reflect the self-calibration provided by monitoring two (or more) emissions. A wide variety of ratiometric sensing probes using small fluorescent molecules have been developed for sensing, imaging, and biomedical applications. In this research highlight, we provide an overview of the design principles underlying small fluorescent probes that have been applied to the ratiometric detection of various analytes, including cations, anions, and biomolecules in solution and in biological samples. This highlight is designed to be illustrative, not comprehensive.
对各种分析物进行定量测定对于从生命科学到环境监测等一系列应用至关重要。光学传感可在生物环境中进行非侵入性测量、对多个样品进行并行监测以及进行侵入性较小的成像。在目前正在探索的光学传感方法中，比率荧光传感作为一种有潜力提供精确和定量分析的技术受到了特别关注。其优点包括高灵敏度和内在可靠性，这反映了通过监测两种（或更多种）发射实现的自校准。已经开发了各种各样使用小荧光分子的比率传感探针用于传感、成像和生物医学应用。在本研究亮点中，我们概述了已应用于溶液和生物样品中各种分析物（包括阳离子、阴离子和生物分子）比率检测的小型荧光探针的设计原理。本亮点旨在具有说明性，而非全面性。
