Smokovitis A A, Kokolis N A, Alexaki E, Binder B R
Department of Physiology, Faculty of Veterinary Medicine, Aristotelian University of Thessaloniki, Greece.
Thromb Res. 1989 Jul 15;55(2):259-65. doi: 10.1016/0049-3848(89)90443-x.
The plasminogen activator activity (PAA) in extracts of the intima, media, and adventitia of the normal human aorta and other large arteries (carotid artery, renal artery and iliac artery) was studied with a sensitive, quantitative spectrophotometric assay using plasminogen and the chromogenic plasmin substrate S-2251. All layers of the arteries showed PAA which was highest in the adventitia, lowest in the media, while in the intima (aorta) PAA was intermediate, but much closer to that of the media. Plasminogen activator inhibition (PAI) was at the same level in all layers of the arteries studied. Plasmin inhibition (PI) was higher in adventitia than in intima (aorta), while in media the PI was intermediate. The PAA was due to the tissue-type plasminogen activator (t-PA), but not to the urokinase-type (u-PA), as judged by addition of respective antibodies. The relatively low PAA found in the intima of large arteries is therefore due to a low plasminogen activator and not a high plasminogen activator inhibitor activity or plasmin inhibitor level.
采用纤溶酶原和发色底物S-2251，通过灵敏的定量分光光度法，研究了正常人体主动脉及其他大动脉（颈动脉、肾动脉和髂动脉）内膜、中膜和外膜提取物中的纤溶酶原激活物活性（PAA）。动脉的所有层均显示有PAA，外膜中最高，中膜中最低，而内膜（主动脉）中的PAA处于中间水平，但更接近中膜。在所研究动脉的所有层中，纤溶酶原激活物抑制物（PAI）处于同一水平。外膜中的纤溶酶抑制物（PI）高于内膜（主动脉），而中膜中的PI处于中间水平。通过加入相应抗体判断，PAA是由组织型纤溶酶原激活物（t-PA）引起，而非尿激酶型（u-PA）。因此，大动脉内膜中相对较低的PAA是由于纤溶酶原激活物含量低，而非纤溶酶原激活物抑制物活性高或纤溶酶抑制物水平高。
