Smokovitis A, Kokolis N, Ploumis T
Department of Physiology, Faculty of Veterinary Medicine, Aristotelian University of Thessaloniki, Greece.
Thromb Res. 1988 May 15;50(4):495-505. doi: 10.1016/0049-3848(88)90198-3.
The effect of endotoxin, aspirin and endotoxin after administration of aspirin on the tissue plasminogen activator activity (PAA), plasminogen activator inhibition (PAI) and plasmin inhibition (PI) was studied in the rat. PAA, PAI and PI were determined in key organs (brain, heart, lungs, kidneys, liver and aorta) spectrophotometrically by procedures involving hydrolysis of the chromogenic substrate S-2251. Aspirin at three different doses had not any significant effect on tissue PAA; PAI and PI were affected in several organs. Four hours after a sustained infusion of endotoxin PAA was found to be increased in brain, kidneys and aorta, decreased in heart and lungs, while in liver the PAA was unchanged compared to controls. Changes in PAI and PI showed also a tissue variation. In endotoxin-infused rats pretreated with aspirin the PAA changes induced by endotoxin were prevented or modified; PAI and PI were affected in most organs studied. However, this effect of aspirin was varying and depending on the tissue, the parameter studied and the dose of aspirin. In some organs, as the heart and lungs, changes in PAI or PI were noticed, while neither aspirin nor endotoxin separately induced such changes in these organs. Also, a differential response of PAI and PI to the same stimulus in the same tissue was a noteworthy finding. The results of the present study show that the response of PAA, PAI and PI to aspirin depends on the tissue, the physiological or pathophysiological condition of the tissue and the dose of the aspirin.
研究了内毒素、阿司匹林以及阿司匹林给药后内毒素对大鼠组织纤溶酶原激活物活性（PAA）、纤溶酶原激活物抑制物（PAI）和纤溶酶抑制物（PI）的影响。通过涉及显色底物S-2251水解的程序，用分光光度法测定关键器官（脑、心脏、肺、肾、肝和主动脉）中的PAA、PAI和PI。三种不同剂量的阿司匹林对组织PAA均无显著影响；PAI和PI在几个器官中受到影响。持续输注内毒素4小时后，发现脑中PAA升高，肾和主动脉中PAA升高，心脏和肺中PAA降低，而肝脏中PAA与对照组相比无变化。PAI和PI的变化也显示出组织差异。在用阿司匹林预处理的内毒素输注大鼠中，内毒素诱导的PAA变化被预防或改变；在大多数研究器官中，PAI和PI受到影响。然而，阿司匹林的这种作用是变化的，取决于组织、所研究的参数和阿司匹林的剂量。在一些器官，如心脏和肺中，观察到PAI或PI的变化，而单独使用阿司匹林或内毒素均未在这些器官中诱导出此类变化。同样，在同一组织中PAI和PI对相同刺激的不同反应也是一个值得注意的发现。本研究结果表明，PAA、PAI和PI对阿司匹林的反应取决于组织、组织的生理或病理生理状况以及阿司匹林的剂量。
