Department of Plant Science, Gangneung-Wonju National University, Gangneung 210-702, Korea.
Department of Biotechnology and Bioengineering, Dongeui University, Busan 614-714, Korea.
Plant Pathol J. 2014 Mar;30(1):102-8. doi: 10.5423/PPJ.OA.05.2013.0052.
The bacterial strain T-9, which shows strong antifungal activity, is isolated from the soils of Samcheok, Gangwondo and identified as Paenibacillus kribbensis according to morphological and taxonomic characteristics and 16S rRNA gene sequence analysis. The P. kribbensis strain T-9 strongly inhibits the growth of various phytopathogenic fungi including Botrytis cinerea, Colletotricum acutatum, Fusarium oxysporum f. sp. radicis-lycopersici, Magnaporthe oryzae, Phytophthora capsici, Rhizoctonia solani, and Sclerotium cepivorum in vitro. Also, the P. kribbensis strain T-9 exhibited similar or better control effects to plant diseases than in fungicide treatment through in vivo assays. In the 2-year greenhouse experiments, P. kribbensis strain T-9 was highly effective against clubroot. In the 2-year field trials, the P. kribbensis strain T-9 was less effective than the fungicide, but reduced clubroot on Chinese cabbage when compared to the control. The above-described results indicate that the strain T-9 may have the potential as an antagonist to control various phytopathogenic fungi.
一株具有较强抗真菌活性的细菌菌株 T-9 从江原道三陟的土壤中分离得到，根据形态学和分类学特征以及 16S rRNA 基因序列分析，鉴定为胶冻样芽孢杆菌。该胶冻样芽孢杆菌 T-9 菌株在体外强烈抑制各种植物病原菌的生长，包括灰葡萄孢、炭疽菌、尖孢镰刀菌、稻瘟病菌、辣椒疫霉、立枯丝核菌和核盘菌。此外，通过体内试验，该胶冻样芽孢杆菌 T-9 菌株在植物病害防治方面表现出与杀菌剂处理相似或更好的控制效果。在为期两年的温室试验中，胶冻样芽孢杆菌 T-9 对根肿病具有高度有效性。在为期两年的田间试验中，胶冻样芽孢杆菌 T-9 的效果不如杀菌剂，但与对照相比，可减少白菜根肿病的发生。上述结果表明，该菌株 T-9 可能具有作为一种拮抗菌来防治各种植物病原菌的潜力。
