聚乳酸/壳聚糖纤维垫：载体对脂肪酶固定化影响的研究
Poly (lactic acid)/chitosan fiber mats: investigation of effects of the support on lipase immobilization.
作者信息
Siqueira Nataly M, Garcia Ketlin C, Bussamara Roberta, Both Fernanda S, Vainstein Marilene H, Soares Rosane M D
机构信息
Laboratory of Advanced Polymers, Institute of Chemistry, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves, 9500, Lab K-208 A, Porto Alegre, RS CEP 91501-970, Brazil; Post-Graduate Program of Materials Science, Universidade Federal do Rio Grande do Sul (UFRGS), Av. Bento Gonçalves, 9500, Sala A216, Porto Alegre, RS CEP 91501-970, Brazil.
Laboratory of Advanced Polymers, Institute of Chemistry, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves, 9500, Lab K-208 A, Porto Alegre, RS CEP 91501-970, Brazil.
出版信息
Int J Biol Macromol. 2015 Jan;72:998-1004. doi: 10.1016/j.ijbiomac.2014.08.048. Epub 2014 Oct 5.
The greatest challenge for biotechnological processes is to have immobilized enzymes acting as good green catalysts with high reusability rates. In this work, we have produced electrospun fibers from poly (lactic acid)/chitosan blends. Further, we evaluated the influence of these materials as support for lipase immobilization. The PLA/chitosan fiber mats were composed by non-woven nanofibers, with diameters ranging from 200 nm to 1.3 μm. The solvent (TFA) as well as the chitosan addition influenced morphology, hydrophobicity and mechanical properties of PLA nanofibers. It was observed that even for lower concentration of lipase (5 U) higher enzyme activity retention was detected in the PLA/chitosan blends. In addition, a remarkable improvement of lipase activity on pure PLA fiber mat was verified, indicating that most of the enzymes were probably in their active form.
生物技术过程面临的最大挑战是使固定化酶成为具有高重复使用率的优质绿色催化剂。在这项工作中，我们用聚乳酸/壳聚糖共混物制备了电纺纤维。此外，我们评估了这些材料作为脂肪酶固定化载体的影响。聚乳酸/壳聚糖纤维毡由直径在200纳米至1.3微米之间的非织造纳米纤维组成。溶剂（三氟乙酸）以及壳聚糖的添加影响了聚乳酸纳米纤维的形态、疏水性和机械性能。据观察，即使对于较低浓度的脂肪酶（5单位），在聚乳酸/壳聚糖共混物中也检测到较高的酶活性保留率。此外，还证实了纯聚乳酸纤维毡上脂肪酶活性有显著提高，这表明大多数酶可能处于活性形式。
Zotero 插件