Kohno S, Watanabe K, Hamamoto A, Dotsu Y, Koga H, Hayashi T, Shigeno Y, Yamaguchi K, Saito A, Hara K
Second Department of Internal Medicine, Nagasaki University School of Medicine.
Tohoku J Exp Med. 1989 Jul;158(3):227-35. doi: 10.1620/tjem.158.227.
Transthoracic needle aspiration of the lung in guinea pigs with experimental pseudomonas pneumonia was evaluated. The number of bacteria in aspirates correlated well with that of bacteria in the lungs which showed diffuse pneumonia (10(7) inoculum group). The number of deaths of experimental animals increased together with an increase of the times of aspiration. This procedure was also investigated in 16 patients of pneumonia and 17 patients of pulmonary abscess. The isolation rate of pathogen from pneumonia was 31.3% and that from pulmonary abscess 58.8%. A higher isolation rate was obtained with purulent aspirates. Predominantly anaerobic bacteria were isolated, and in pulmonary abscess usually in association with other bacteria. This method could be applied with success, for determining responsible pathogens. Even the normal oropharyngeal flora such as alpha-Streptococcus could be identified as pathogens. The complication rate was relatively low (6 out of 33 patients, 18.2%) including hemoptysis as a major one and pneumothorax or bloody sputum as minor ones. Transthoracic needle aspiration was reevaluated in experimental and clinical materials and was found to be an excellent and safe method for determining the responsible pathogen of respiratory infection.
对患有实验性铜绿假单胞菌肺炎的豚鼠进行经胸肺穿刺抽吸术进行了评估。抽吸物中的细菌数量与肺部出现弥漫性肺炎的细菌数量（接种量为10⁷的组）密切相关。实验动物的死亡数量随着抽吸次数的增加而增加。还对16例肺炎患者和17例肺脓肿患者进行了该操作。肺炎患者病原体的分离率为31.3%，肺脓肿患者为58.8%。脓性抽吸物的分离率更高。主要分离出厌氧菌，在肺脓肿中通常与其他细菌并存。该方法可成功用于确定致病病原体。甚至正常的口咽菌群如α-链球菌也可被鉴定为病原体。并发症发生率相对较低（33例患者中有6例，18.2%），主要并发症为咯血，次要并发症为气胸或血痰。经对实验和临床资料重新评估，经胸肺穿刺抽吸术被发现是确定呼吸道感染致病病原体的一种优良且安全的方法。
