Holme S, Moroff G, Whitley P, Hallinen D, Heaton A
American Red Cross and Eastern Virginia Medical School, Norfolk.
Transfusion. 1989 Oct;29(8):689-92. doi: 10.1046/j.1537-2995.1989.29890020441.x.
Extension of the maximum holding time for whole blood collected into a CPD-ADSOL system from 6 to 8 hours at ambient temperature under conditions that cause the temperature of the blood to decrease to 20 to 24 degrees C would facilitate the preparation of platelet concentrates (PCs). In this study, the properties of CPD-PCs prepared and stored for 5 days in PL-732 containers after various initial holding periods were assessed in two laboratories, designated Laboratory A and Laboratory B. Laboratory A found higher platelet-rich plasma (PRP) volumes (276 +/- 25 vs. 249 +/- 19 mL) and platelet yields (76 +/- 18 vs. 66 +/- 18 x 10(9) platelets) with 8-hour holds (n = 10) than with 1- to 2-hour holds (n = 10), although only the difference in PRP volumes was significant (p less than 0.05). No significant difference was observed in autologous in vivo recovery (54 +/- 11 vs. 47 +/- 9%) or survival (167 +/- 37 vs. 170 +/- 25 hrs), as calculated by gamma function using 111In as radiolabel. Laboratory B also found higher PRP volumes (304 +/- 31 vs. 279 +/- 37 mL) and platelet yields with 8-hour holds (n = 12) than with a 6-hour holds (n = 10) (88 +/- 26 vs. 77 +/- 27 x 10(9) platelets). No significant differences were found in morphology score, the extent of release of beta-thromboglobulin, the discharge of lactate dehydrogenase, or hypotonic shock response.(ABSTRACT TRUNCATED AT 250 WORDS)
在环境温度下，将收集到CPD - ADSOL系统中的全血最长保存时间从6小时延长至8小时，且使血液温度降至20至24摄氏度的条件下，将有助于血小板浓缩物（PCs）的制备。在本研究中，在两个实验室（分别指定为实验室A和实验室B）对在不同初始保存期后于PL - 732容器中制备并储存5天的CPD - PCs的特性进行了评估。实验室A发现，8小时保存（n = 10）时的富血小板血浆（PRP）体积（276±25 vs. 249±19 mL）和血小板产量（76±18 vs. 66±18×10⁹血小板）高于1至2小时保存（n = 10）时，尽管只有PRP体积的差异具有统计学意义（p＜0.05）。使用¹¹¹In作为放射性标记通过γ函数计算，自体体内回收率（54±11 vs. 47±9%）或存活率（167±37 vs. 170±25小时）未观察到显著差异。实验室B也发现，8小时保存（n = 12）时的PRP体积（304±31 vs. 279±37 mL）和血小板产量高于6小时保存（n = 10）时（88±26 vs. 77±27×10⁹血小板）。在形态学评分、β - 血小板球蛋白释放程度、乳酸脱氢酶释放量或低渗休克反应方面未发现显著差异。（摘要截断于250字）
