冬凌草甲素通过调节非肌肉肌球蛋白IIA抑制细胞迁移。
Oridonin suppress cell migration via regulation of nonmuscle myosin IIA.
作者信息
Li Yin-Chao, Sun Mo-Ran, Zhao Yi-Hong, Fu Xian-Zu, Xu Hai-Wei, Liu Ji-Feng
机构信息
School of Pharmaceutical Sciences, Zhengzhou University, 100 Kexue Avenue, Zhengzhou, 450001, People's Republic of China.
出版信息
Cytotechnology. 2016 May;68(3):389-97. doi: 10.1007/s10616-014-9790-4. Epub 2014 Oct 9.
Abstract
Oridonin, which is isolated from Chinese herb Rabdosia rubescens (Hemsl.) Hara, has been implicated in regulation of tumor cell migration and invasion. In this study, treatment with oridonin enhanced the phosphorylation of myosin regulatory light chain (T18/S19) that regulates the ATPase activity of myosin IIA. Meanwhile, stress fibers were significantly more prominent after oridonin incubation, which impaired cell migration in transwell migration assays. All of these effects may be caused by the decreased interaction between myosin IIA and myosin phosphatase complex, but not kinases. Our data provide clear evidence of this novel pharmacological function for oridonin in treating cancer cell migration.
摘要
冬凌草甲素是从中药冬凌草中分离得到的，它与肿瘤细胞的迁移和侵袭调控有关。在本研究中，冬凌草甲素处理增强了肌球蛋白调节轻链（T18/S19）的磷酸化，该轻链调节肌球蛋白IIA的ATP酶活性。同时，经冬凌草甲素孵育后应力纤维明显更突出，这在transwell迁移实验中损害了细胞迁移。所有这些效应可能是由肌球蛋白IIA与肌球蛋白磷酸酶复合物之间相互作用的减少引起的，而非激酶。我们的数据为冬凌草甲素在治疗癌细胞迁移方面的这种新的药理功能提供了明确证据。
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