肠道病毒的分子特征，包括2011年从中国山东的新疆学生中分离出的新型肠道病毒C99型。

Molecular characterization of enteroviruses including a new type EV-C99 isolated from Xinjiang students in Shandong, China in 2011.

作者信息

Tao Zexin, Yuan Qun, Lin Xiaojuan, Wang Suting, Liu Yao, Ji Feng, Xiong Ping, Cui Ning, Song Lizhi, Wang Mei, Xu Aiqiang

机构信息

1] Academy of Preventive Medicine, Shandong University, Jinan, People's Republic of China [2] Shandong Provincial Key Laboratory of Infectious Disease Control and Prevention, Shandong Center for Disease Control and Prevention, Jinan, People's Republic of China.

Department of Preventive Medicine, College of Basic Medical Sciences, Shandong University of Traditional Chinese Medicine, Jinan, People's Republic of China.

出版信息

Sci Rep. 2014 Oct 9;4:6564. doi: 10.1038/srep06564.

DOI:10.1038/srep06564

PMID:25298041

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4190507/

Abstract

The last case of infection with wild-type poliovirus indigenous to China was reported in 1994. In 2011, a poliomyelitis outbreak caused by imported wide-type poliovirus occurred in Xinjiang Uighur Autonomous Region. Here, we report the results of enterovirus (EV) isolation from Xinjiang students that returned to school in Shandong after summer vacation during this outbreak. Stool specimens from 376 students were collected and 10 EV strains were isolated including 4 polioviruses (All Sabin strains), 1 coxsackievirus (CV) A13, 3 CVA17 and 2 EV-C99. VP1 sequence analysis revealed these CVA13, CVA17 and EV-C99 strains had 71.3-81.8%, 76.5-84.6% and 74.2-82.9% nucleotide similarity with strains from other countries within a serotype, respectively. EV-C99 strains had 82.7-92.8% VP1 similarity with two previously reported Xinjiang strains. Complete genome analysis on EV-C99 strains revealed intra-serotypic genetic recombination events. These findings reflect great genetic divergence between Chinese strains and strains from other countries of the three types, and provide valuable information on monitoring EV transmission over long distance.

摘要

中国本土野生型脊髓灰质炎病毒的最后一例感染病例于1994年被报告。2011年，新疆维吾尔自治区发生了由输入性野生型脊髓灰质炎病毒引起的脊髓灰质炎疫情。在此，我们报告了此次疫情期间暑假后返回山东上学的新疆学生肠道病毒（EV）分离结果。收集了376名学生的粪便标本，分离出10株EV毒株，包括4株脊髓灰质炎病毒（均为Sabin株）、1株柯萨奇病毒（CV）A13、3株CVA17和2株EV-C99。VP1序列分析显示，这些CVA13、CVA17和EV-C99毒株与血清型内其他国家的毒株核苷酸相似性分别为71.3%-81.8%、76.5%-84.6%和74.2%-82.9%。EV-C99毒株与之前报告的两株新疆毒株VP1相似性为82.7%-92.8%。对EV-C99毒株的全基因组分析揭示了血清型内的基因重组事件。这些发现反映了这三种类型的中国毒株与其他国家毒株之间存在很大的遗传差异，并为远距离监测EV传播提供了有价值的信息。