2012 年中国新肠道病毒 EV-B106 的全基因组特征分析。
Complete genome characterization of a novel enterovirus type EV-B106 isolated in China, 2012.
机构信息
1] Yunnan Center for Disease Control and Prevention, Kunming, Yunnan Province, People's Republic of China [2].
1] Shandong Provincial Key Laboratory of Infectious Disease Control and Prevention, Shandong Center for Disease Control and Prevention, Jinan, Shandong Province, People's Republic of China [2].
出版信息
Sci Rep. 2014 Mar 3;4:4255. doi: 10.1038/srep04255.
Human enterovirus B106 (EV-B106) is a recently identified member of enterovirus species B. In this study, we report the complete genomic characterization of an EV-B106 strain (148/YN/CHN/12) isolated from an acute flaccid paralysis patient in Yunnan Province, China. The new strain had 79.2-81.3% nucleotide and 89.1-94.8% amino acid similarity in the VP1 region with the other two EV-B106 strains from Bolivia and Pakistan. When compared with other EV serotypes, it had the highest (73.3%) VP1 nucleotide similarity with the EV-B77 prototype strain CF496-99. However, when aligned with all EV-B106 and EV-B77 sequences available from the GenBank database, two major frame shifts were observed in the VP1 coding region, which resulted in substantial (20.5%) VP1 amino acid divergence between the two serotypes. Phylogenetic analysis and similarity plot analysis revealed multiple recombination events in the genome of this strain. This is the first report of the complete genome of EV-B106.
肠道病毒 B106 型（EV-B106）是人肠道病毒 B 组的一个新成员。本研究报道了一株从中国云南省急性弛缓性麻痹患者中分离到的 EV-B106 新病毒（148/YN/CHN/12）的全基因组特征。该新分离株与来自玻利维亚和巴基斯坦的另外两株 EV-B106 分离株在 VP1 区的核苷酸序列同源性为 79.2-81.3%，氨基酸序列同源性为 89.1-94.8%。与其他肠道病毒血清型相比，与 EV-B77 原型株 CF496-99 的 VP1 核苷酸序列同源性最高（73.3%）。然而，与 GenBank 数据库中所有可获得的 EV-B106 和 EV-B77 序列进行比对时，在 VP1 编码区观察到两个主要的移框，导致两个血清型之间的 VP1 氨基酸差异较大（20.5%）。系统进化分析和相似性图谱分析显示该病毒株基因组中存在多个重组事件。这是首次报道 EV-B106 的全基因组序列。
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