Zhang Keyi, Hong Mei, Zhang Yong, Han Zhenzhi, Xiao Jinbo, Lu Huanhuan, Song Yang, Yan Dongmei, Wang Dongyan, Zhu Shuangli, Xu Wenbo, Wu Guizhen
WHO WPRO Regional Polio Reference Laboratory, NHC Key Laboratory for Biosafety, NHC Key Laboratory for Medical Virology, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China.
Tibet Center for Disease Control and Prevention, Lhasa, China.
Front Microbiol. 2021 Jan 13;11:632552. doi: 10.3389/fmicb.2020.632552. eCollection 2020.
Enterovirus B75 (EV-B75) is a newly identified serotype of the enterovirus B species. To date, only 112 cases related to EV-B75 have been reported worldwide, and research on EV-B75 is still limited with only two full-length genome sequences available in GenBank. The present study reported seven EV-B75 sequences from a child with acute flaccid paralysis and six asymptomatic close contacts in Shigatse, Tibet. Phylogenetic analysis revealed that the Tibetan strain was possibly imported from neighboring India. Seroepidemiological analyses indicated that EV-B75 has not yet caused a large-scale epidemic in Tibet. Similarity plots and boot scanning analyses revealed frequent intertypic recombination in the non-structural region of all seven Tibet EV-B75 strains. All seven Tibetan strains were temperature-sensitive, suggesting their poor transmissibility in the environment. Overall, though the seven Tibetan strains did not cause large-scale infection, prevention and control of the novel enterovirus cannot be underestimated.
肠道病毒B75（EV-B75）是新发现的肠道病毒B种血清型。迄今为止，全球仅报告了112例与EV-B75相关的病例，对EV-B75的研究仍然有限，GenBank中仅有两条全长基因组序列。本研究报告了来自西藏日喀则一名急性弛缓性麻痹儿童及六名无症状密切接触者的7条EV-B75序列。系统发育分析表明，西藏毒株可能是从邻国印度传入的。血清流行病学分析表明，EV-B75尚未在西藏引起大规模流行。相似性图谱和Boot扫描分析显示，所有7株西藏EV-B75毒株的非结构区域存在频繁的型间重组。所有7株西藏毒株均对温度敏感，表明它们在环境中的传播能力较差。总体而言，尽管这7株西藏毒株未引起大规模感染，但新型肠道病毒的防控工作仍不容小觑。
