利用酿酒废水和生活污水混合培养的产油酵母和微藻生产油脂。
Lipid production by a mixed culture of oleaginous yeast and microalga from distillery and domestic mixed wastewater.
机构信息
Department of Civil and Environmental Engineering, Faculty of Science and Technology, University of Macau, Taipa, Macau SAR, China.
Department of Civil and Environmental Engineering, Faculty of Science and Technology, University of Macau, Taipa, Macau SAR, China.
出版信息
Bioresour Technol. 2014 Dec;173:132-139. doi: 10.1016/j.biortech.2014.09.047. Epub 2014 Sep 22.
Lipid productivity by mixed culture of Rhodosporidium toruloides and Chlorella pyrenoidosa was studied using 1:1 mixed real wastewater from distillery and local municipal wastewater treatment plant with initial soluble chemical oxygen demand (SCOD) around 25,000 mg/L, initial cell density of 2×10(7) cells/mL (yeast) and 5×10(6) cells/mL (microalga), at 30 °C and 2.93 W/m2 (2000 lux, 12:12 h light and dark cycles). Lipid content and lipid yield achieved were 63.45±2.58% and 4.60±0.36 g/L with the associated removal efficiencies for SCOD, total nitrogen (TN), and total phosphorus (TP) at 95.34±0.07%, 51.18±2.17%, and 89.29±4.91%, respectively, after 5 days of cultivation without the pH adjustment. Inoculation of microalgae at 40 h of the initial yeast cultivation and harvesting part of inactive biomass at 72 h by sedimentation could improve both lipid production and wastewater treatment efficiency under non-sterile conditions.
采用初始可溶性化学需氧量（SCOD）约为 25000mg/L、初始细胞密度为 2×10(7)cells/mL（酵母）和 5×10(6)cells/mL（微藻）的 1:1 混合真实酿酒废水和当地城市污水处理厂混合废水，在 30°C 和 2.93 W/m2（2000lux，12:12h 光照和黑暗周期）下，研究了粘红酵母（Rhodosporidium toruloides）和蛋白核小球藻（Chlorella pyrenoidosa）混合培养的产脂情况。在不调节 pH 值的情况下，培养 5 天后，可分别实现 63.45±2.58%和 4.60±0.36g/L 的脂质含量和脂质产率，同时对 SCOD、总氮（TN）和总磷（TP）的去除效率分别达到 95.34±0.07%、51.18±2.17%和 89.29±4.91%。在初始酵母培养 40h 时接种微藻，并在 72h 时通过沉淀收获部分失活生物质，可以在非无菌条件下提高脂质生产和废水处理效率。
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