嗜纤维微杆菌21对多唾液酸神经节苷脂进行高效生物转化以制备GM1 。
Efficient biotransformation of polysialogangliosides for preparation of GM1 by Cellulosimicrobium sp. 21.
作者信息
Zheng Yan, Ji Li, Leng Jiayi, Yuan Ye, Chen Honglei, Gou Dongxia, Gao Yufei, Zhou Yifa
机构信息
China-Japan Union Hospital, Jilin University, Changchun 130033, Jilin, China.
School of Life Sciences, Northeast Normal University, Changchun 130024, Jilin, China.
出版信息
Molecules. 2014 Oct 8;19(10):16001-12. doi: 10.3390/molecules191016001.
A new ganglioside transformed strain isolated from soil was identified as Cellulosimicrobium sp. 21. It produced a sialidase which transformed polysialo-gangliosides GD1 and GT1 into a monosialoterahexosylganglioside, i.e., ganglioside GM1. The sialidase had both NeuAc-α-2,3- and NeuAc-α-2,8-sialidase activity without producing asiolo-GM1. The optimum conditions were evaluated and it was found that the transformation was optimally performed at 30 °C and pH 7.0. The substrate should be added at the beginning of the reaction and the concentration of substrate was 3% (w/v). Under these optimum conditions, Cellulosimicrobium sp. 21 converted GD1 and GT1 into GM1 in inorganic medium in a 5 L bioreactor with the recovery rate of 69.3%. The product contained 50.3% GM1 and was purified on silica to give the product with 95% of GM1 with a recovery rate of 30.5%. Therefore, Cellulosimicrobium sp. 21 has potential to be applied in the production of GM1 in the pharmaceutical industry.
从土壤中分离出的一株新的神经节苷脂转化菌株被鉴定为纤维微菌属菌株21。它产生一种唾液酸酶，可将多唾液酸神经节苷脂GD1和GT1转化为单唾液酸四己糖神经节苷脂，即神经节苷脂GM1。该唾液酸酶同时具有NeuAc-α-2,3-和NeuAc-α-2,8-唾液酸酶活性，且不产生唾液酸GM1。对最佳条件进行了评估，发现转化反应在30℃和pH 7.0时效果最佳。底物应在反应开始时添加，底物浓度为3%（w/v）。在这些最佳条件下，纤维微菌属菌株21在5 L生物反应器的无机培养基中将GD1和GT1转化为GM1，回收率为69.3%。产物中含有50.3%的GM1，经硅胶纯化后得到GM1含量为95%的产物，回收率为30.5%。因此，纤维微菌属菌株21有潜力应用于制药行业GM1的生产。
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