College of Plant Protection, Nanjing Agricultural University, Key Laboratory of Pesticide, Nanjing, Jiangsu Province, 210095, China.
Plant Dis. 2020 Oct;104(10):2696-2703. doi: 10.1094/PDIS-10-19-2253-RE. Epub 2020 Jul 30.
In present study, the morphological and physiological characteristics of (Lib.) de Bary to a novel succinate dehydrogenase inhibitor (SDHI) fungicide penthiopyrad has been reported. The baseline sensitivity of to penthiopyrad was determined using 119 strains by inhibition of mycelial growth. The median effective concentration (EC) values for penthiopyrad ranged from 0.0096 to 0.2606 μg/ml, and the mean value was 0.0578 (±0.0626) μg/ml. After 1 μg/ml penthiopyrad treatment, mycelia of strains showed increased apical branching and were denser compared with control, and cell membrane permeability significantly increased. In addition, glycerol content, oxalic acid (OA), and exopolysaccharide (EPS) content decreased markedly and mycelial respiration was distinctly inhibited. The number and dry weight of sclerotia significantly decreased after being treated with 2 μg/ml penthiopyrad. Penthiopyrad exhibited both protective and curative activity on the detached rapeseed leaves. Importantly, the above results will provide us more information on penthiopyrad for management of diseases caused by and increase our understanding of action of penthiopyrad against .
本研究报道了（Lib.）de Bary 对一种新型琥珀酸脱氢酶抑制剂（SDHI）杀菌剂戊吡丙醚的形态和生理特性。采用菌丝生长抑制法测定了 119 株对戊吡丙醚的基础敏感性。戊吡丙醚对 的 EC50 值范围为 0.0096 至 0.2606μg/ml，平均值为 0.0578（±0.0626）μg/ml。经 1μg/ml 戊吡丙醚处理后，与对照相比， 菌株的菌丝顶端分枝增加，密度增加，细胞膜通透性显著增加。此外，甘油含量、草酸（OA）和胞外多糖（EPS）含量明显下降，菌丝呼吸明显受到抑制。用 2μg/ml 戊吡丙醚处理后，菌核的数量和干重显著减少。戊吡丙醚对离体油菜叶片具有保护和治疗活性。重要的是，上述结果将为我们提供更多关于戊吡丙醚防治引起的病害的信息，并增加我们对戊吡丙醚防治的作用机制的理解。
