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在巴西对艰难梭菌核糖体分型027和078进行的一项调查。

A search for Clostridium difficile ribotypes 027 and 078 in Brazil.

作者信息

Monteiro Alexandre de Almeida, Pires Renata Neto, Persson Søren, Rodrigues Filho Edison Moraes, Pasqualotto Alessandro Comarú

机构信息

PPG Patologia, Universidade Federal de Ciências da Saúde de Porto Alegre, Porto Alegre, RS, Brazil.

PPG Patologia, Universidade Federal de Ciências da Saúde de Porto Alegre, Porto Alegre, RS, Brazil; Santa Casa de Misericórdia de Porto Alegre, Porto Alegre, RS, Brazil.

出版信息

Braz J Infect Dis. 2014 Nov-Dec;18(6):672-4. doi: 10.1016/j.bjid.2014.08.004. Epub 2014 Oct 11.

DOI:10.1016/j.bjid.2014.08.004
PMID:25307680
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9425211/
Abstract

Toxigenic strains of Clostridium difficile may be disseminating. Here we prospectively screened patients with nosocomial diarrhoea in two hospitals in Brazil. To identify C. difficile polymerase chain reaction ribotypes 027/078 strains, we used high resolution melting and multiplex polymerase chain reaction. Among 116 screened patients, 11 were positive for C. difficile. The polymerase chain reaction ribotypes 027/078 strains were not identified in this study.

摘要

艰难梭菌的产毒菌株可能正在传播。在此，我们对巴西两家医院的医院获得性腹泻患者进行了前瞻性筛查。为鉴定艰难梭菌聚合酶链反应核糖体分型027/078菌株，我们使用了高分辨率熔解曲线分析和多重聚合酶链反应。在116名接受筛查的患者中，11名艰难梭菌检测呈阳性。本研究未鉴定出聚合酶链反应核糖体分型027/078菌株。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8adb/9425211/05f79362ace6/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8adb/9425211/bb6e2d712543/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8adb/9425211/05f79362ace6/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8adb/9425211/bb6e2d712543/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8adb/9425211/05f79362ace6/gr2.jpg

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J Microbiol Methods. 2012 May;89(2):87-94. doi: 10.1016/j.mimet.2012.02.011. Epub 2012 Mar 3.
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Multiplex PCR method for detection of Clostridium difficile tcdA, tcdB, cdtA, and cdtB and internal in-frame deletion of tcdC.
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J Clin Microbiol. 2011 Dec;49(12):4299-300. doi: 10.1128/JCM.05161-11. Epub 2011 Oct 5.
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Comparison of strain typing results for Clostridium difficile isolates from North America.比较北美分离的艰难梭菌的菌株分型结果。
J Clin Microbiol. 2011 May;49(5):1831-7. doi: 10.1128/JCM.02446-10. Epub 2011 Mar 9.
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Detection of cross-infection associated to a Brazilian PCR-ribotype of Clostridium difficile in a university hospital in Rio de Janeiro, Brazil.巴西里约热内卢一所大学医院中与一种巴西 PCR 型艰难梭菌相关的交叉感染检测。
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