Nayak Sushma S, Ankola Anil V, Metgud Sharda C, Bolmal Uday Kumar
Senior Lecturer and Research Scholar, Department of Public Health Dentistry, KLE VK Institute of Dental Sciences, KLE University, Belgaum, Karnataka, India e-mail:
Professor and Head, Department of Public Health Dentistry, KLE VK Institute of Dental Sciences, KLE University, Belgaum, Karnataka, India.
J Contemp Dent Pract. 2014 May 1;15(3):278-82. doi: 10.5005/jp-journals-10024-1528.
Many weapons are available in the arsenal of a dental professional to combat dental caries, which is almost ubiquitously present. From a public health perspective, most of these weapons are far from being an ideal drug. Hence, there is a demand for better and effective antibacterial agents. This factor stimulated the process of the present study. The aim of the study was to determine the effect of ethanol extract of Terminalia chebula on Streptococcus mutans.
Dried ripe fruits of Terminalia chebula were procured and powdered. Physical tests were done to estimate purity of the fruit powder. Hydroethanolic and aqueous extracts were prepared according to standard procedures. Minimum inhibitory concentration of the extracts was determined by tube dilution method and confirmed by agar dilution method. The effect of the hydroethanolic extract on sucrose induced adhesion, glucan-induced aggregation and on glycolysis of Streptococcus mutans was also assessed. Preservative, gelling agent and sweetener were added in suitable quantities to the ethanol extract, and mouthrinse was formulated. Minimum inhibitory concentration of the formulation was also determined.
Yield was better in case of aqueous extract. The Minimum inhibitory concentration of hydroethanolic extract was determined to be 2.5%. Minimum inhibitory concentration of the aqueous extract was determined to be 10%. Hydroethanolic extract of Terminalia chebula (2.5%) inhibited sucrose induced adherence and aggregation of Streptococcus mutans in vitro.
The mouthrinse formulated from ethanol extract of Terminalia chebula demonstrated substantial antibacterial activity and could be used as an effective anticaries agent.
Terminalia chebula mouthrinse can be effectively used in clinical practice as an anticaries mouthrinse with additional benefit being that it is safe and economical.
在牙科专业人员对抗几乎普遍存在的龋齿的“武器库”中有许多手段。从公共卫生角度来看，这些手段大多远非理想药物。因此，需要更好且有效的抗菌剂。这一因素推动了本研究进程。本研究的目的是确定诃子乙醇提取物对变形链球菌的作用。
采购诃子干燥成熟果实并磨成粉末。进行物理测试以评估果粉纯度。按照标准程序制备水乙醇提取物和水提取物。通过试管稀释法测定提取物的最低抑菌浓度，并通过琼脂稀释法进行确认。还评估了水乙醇提取物对蔗糖诱导的黏附、葡聚糖诱导的聚集以及对变形链球菌糖酵解的影响。向乙醇提取物中适量添加防腐剂、胶凝剂和甜味剂，配制漱口水。还测定了该制剂的最低抑菌浓度。
水提取物的产率更高。水乙醇提取物的最低抑菌浓度测定为2.5%。水提取物的最低抑菌浓度测定为10%。诃子水乙醇提取物（2.5%）在体外抑制了蔗糖诱导的变形链球菌黏附和聚集。
由诃子乙醇提取物配制的漱口水显示出显著的抗菌活性，可作为有效的防龋剂使用。
诃子漱口水可有效用于临床实践作为防龋漱口水，另外其安全性高且经济实惠。
