Department of Biomedical Laboratory Science, College of Health Sciences, Eulji University, Seongnam, Republic of Korea.
Department of Dental Hygiene, College of Health Science, Eulji University, Seongnam, Republic of Korea.
Clin Exp Dent Res. 2021 Dec;7(6):987-994. doi: 10.1002/cre2.467. Epub 2021 Jun 28.
Dental caries is a high prevalent chronic bacterial infectious disease caused by plaque, a bacterial colony deposited on tooth surfaces and gum tissues. Streptococcus mutans is a primary cariogenic bacterium commonly found in the human oral cavity. Oral hygiene products containing antibacterial ingredients can be helpful in caries management. In this study, we investigated the anticaries mechanism of the ethanol extract of Terminalia chebula (EETC) on S. mutans and suggest its possible application as a functional ingredients for oral hygiene products.
The EETC was prepared from the Terminalia chebula fruit. Disk diffusion, minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), and colony forming unit (CFU) were analyzed to observe the antibacterial activity of EETC. The glucan formation was measured using the filtrate of bacterial culture medium and sucrose. Gene expression was analyzed using RT-PCR. Cytotoxicity was analyzed using the MTT assay. The radical-scavenging activities of DPPH and ABTS were also tested to verify the antioxidant activity of EETC.
The antibacterial activity of the EETC was explored through a disc diffusion analysis and CFU measurement. EETC treatment decreased insoluble glucan formation and gene expression of glycosyltransferase B (gtf B), glycosyltransferase C (gtf C), glycosyltransferase D (gtf D), and fructosyltransferase (ftf). The MIC and MBC of EETC on S. mutans were not cytotoxic to gingival fibroblasts. In addition, we observed DPPH and ABTS-radical scavenging activities of EETC.
These results indicate that the antibacterial and antioxidant effects of EETC may contribute to oral hygiene products for dental caries management.
龋齿是一种由菌斑引起的高发性慢性细菌性传染病，菌斑是一种沉积在牙齿表面和牙龈组织上的细菌菌落。变形链球菌是口腔中常见的主要致龋细菌。含有抗菌成分的口腔卫生用品有助于龋齿的管理。在这项研究中，我们研究了诃子乙醇提取物（EETC）对变形链球菌的抗龋机制，并提出将其作为口腔卫生用品的功能性成分的可能性。
从诃子果实中提取 EETC。采用圆盘扩散法、最小抑菌浓度（MIC）、最小杀菌浓度（MBC）和菌落形成单位（CFU）分析来观察 EETC 的抗菌活性。采用细菌培养液滤液和蔗糖测量葡聚糖的形成。使用 RT-PCR 分析基因表达。采用 MTT 测定法分析细胞毒性。还测试了 DPPH 和 ABTS 的自由基清除活性，以验证 EETC 的抗氧化活性。
通过圆盘扩散分析和 CFU 测量探索了 EETC 的抗菌活性。EETC 处理降低了不溶性葡聚糖的形成和糖基转移酶 B（gtf B）、糖基转移酶 C（gtf C）、糖基转移酶 D（gtf D）和果糖基转移酶（ftf）的基因表达。EETC 对变形链球菌的 MIC 和 MBC 对牙龈成纤维细胞没有细胞毒性。此外，我们观察到 EETC 对 DPPH 和 ABTS 自由基的清除活性。
这些结果表明，EETC 的抗菌和抗氧化作用可能有助于口腔卫生用品管理龋齿。
