神经肌肉健康受试者椎旁肌的组织学特征及生化分析
Histological characterization and biochemical analysis of paraspinal muscles in neuromuscularly healthy subjects.
作者信息
Zimmermann Claudia, Kalepu Rajakiran, Ponfick Matthias, Reichel Heiko, Cakir Balkan, Zierz Stephan, Gdynia Hans-Jürgen, Kassubek Jan, Ludolph Albert C, Rosenbohm Angela
机构信息
Department of Neurology, University of Ulm, Oberer Eselsberg 45, 89081, Ulm, Germany.
Department of Orthopaedic Surgery, University of Ulm, Ulm, Germany.
出版信息
Muscle Nerve. 2015 Jul;52(1):45-54. doi: 10.1002/mus.24490. Epub 2015 May 28.
INTRODUCTION
There are no generally accepted histopathological reference values in paraspinal skeletal muscle biopsies.
METHODS
We examined multifidii muscle biopsies from 20 neuromuscularly healthy subjects using routine histological stains and biochemical analyses of respiratory chain enzymes.
RESULTS
Staining showed incomplete myopathic features, such as increased variability in fiber size, type 1 hypertrophy, rounded fiber shape, endomysial fibrosis, and replacement by adipose tissue. Acid phosphatase reaction was positive in up to 35% of the selected muscle fibers. Mitochondrial changes were obvious but revealed no selective age dependence. Reduced complex I, cytochrome c oxidase (COX), and citrate synthase (CS) could be observed.
CONCLUSIONS
Because the increased variability in morphological details can easily be misinterpreted as myopathic changes, analysis of paraspinal muscles should take into consideration that incomplete myopathic features and reduced oxidative enzyme activities for complex I, COX, and CS are normal variations at this location.
引言
目前尚无普遍接受的脊柱旁骨骼肌活检组织病理学参考值。
方法
我们使用常规组织学染色和呼吸链酶的生化分析，对20名神经肌肉健康受试者的多裂肌活检样本进行了检查。
结果
染色显示出不完全的肌病特征，如纤维大小变异性增加、1型纤维肥大、纤维形状变圆、肌内膜纤维化以及被脂肪组织替代。酸性磷酸酶反应在高达35%的所选肌纤维中呈阳性。线粒体变化明显，但未显示出与年龄的选择性相关性。可观察到复合物I、细胞色素c氧化酶（COX）和柠檬酸合酶（CS）减少。
结论
由于形态细节的变异性增加很容易被误解为肌病性改变，因此在分析脊柱旁肌肉时应考虑到不完全的肌病特征以及复合物I、COX和CS的氧化酶活性降低是该部位的正常变异。
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