Nemeth P M
Muscle Nerve. 1982 Feb;5(2):134-9. doi: 10.1002/mus.880050210.
The influence of muscular contraction on the oxidative enzymes and the diameters of muscle fibers was investigated. Soleus muscles of guinea pigs were denervated for four weeks. The denervated fibers showed a reduction in the intensity of staining for beta-hydroxybutyrate dehydrogenase, cytochrome oxidase, succinate dehydrogenase, and NADH-dependent tetrazolium reductase. Denervation also resulted in a decrease in fiber diameter. Denervated soleus muscles were electrically stimulated to contract over a four-week period at a frequency normally received by slow contracting muscles. Electrical stimulation caused the stain intensity of histochemical reactions for oxidative enzymes to appear to be normal or greater than normal in 90% of the denervated fibers. Stimulation also caused 69% of the denervated fibers to be of normal or greater than normal size. The results demonstrate that contraction of denervated muscle by electrical stimulation prevents the loss of oxidative enzymes and the atrophy associated with denervation.
研究了肌肉收缩对氧化酶和肌纤维直径的影响。将豚鼠的比目鱼肌去神经支配四周。去神经支配的纤维显示β-羟丁酸脱氢酶、细胞色素氧化酶、琥珀酸脱氢酶和NADH依赖性四唑还原酶的染色强度降低。去神经支配还导致纤维直径减小。对去神经支配的比目鱼肌进行电刺激，使其在四周内以慢收缩肌肉通常接受的频率收缩。电刺激使90%的去神经支配纤维的氧化酶组织化学反应染色强度看起来正常或高于正常。刺激还使69%的去神经支配纤维大小正常或大于正常。结果表明，通过电刺激使去神经支配的肌肉收缩可防止氧化酶的丧失以及与去神经支配相关的萎缩。
