Lewinska Anna, Adamczyk Jagoda, Pajak Justyna, Stoklosa Sylwia, Kubis Barbara, Pastuszek Paulina, Slota Ewa, Wnuk Maciej
Department of Biochemistry and Cell Biology, University of Rzeszow, Poland; Centre of Applied Biotechnology and Basic Sciences, University of Rzeszow, Kolbuszowa, Poland.
Centre of Applied Biotechnology and Basic Sciences, University of Rzeszow, Kolbuszowa, Poland; Department of Genetics, University of Rzeszow, Rejtana 16C, 35-959 Rzeszow, Poland.
Mutat Res Genet Toxicol Environ Mutagen. 2014 Sep 1;771:43-52. doi: 10.1016/j.mrgentox.2014.07.001. Epub 2014 Jul 9.
Curcumin, the major yellow-orange pigment of turmeric derived from the rhizome of Curcuma longa, is a highly pleiotropic molecule with the potential to modulate inflammation, oxidative stress, cell survival, cell secretion, homeostasis and proliferation. Curcumin, at relatively high concentrations, was repeatedly reported to be a potent inducer of apoptosis in cancer cells and thus considered a promising anticancer agent. In the present paper, the effects of low concentrations of curcumin on human cervical cancer (HeLa) cells were studied. We found curcumin-mediated decrease in the cell number and viability, and increase in apoptotic events and superoxide level. In contrast to previously shown curcumin cytotoxicity toward different cervical cancer lines, we observed toxic effects when even as low as 1 μM concentration of curcumin was used. Curcumin was not genotoxic to HeLa cells. Because argyrophilic nucleolar protein (AgNOR protein) expression is elevated in malignant cells compared to normal cells reflecting the rapidity of cancer cell proliferation, we evaluated curcumin-associated changes in size (area) and number of silver deposits. We showed curcumin-induced decrease in AgNOR protein pools, which may be mediated by global DNA hypermethylation observed after low concentration curcumin treatment. In summary, we have shown for the first time that curcumin at low micromolar range may be effective against HeLa cells, which may have implications for curcumin-based treatment of cervical cancer in humans.
姜黄素是从姜黄根茎中提取的主要黄橙色色素，是一种具有高度多效性的分子，有调节炎症、氧化应激、细胞存活、细胞分泌、体内平衡和增殖的潜力。据多次报道，较高浓度的姜黄素是癌细胞凋亡的有效诱导剂，因此被认为是一种有前景的抗癌药物。在本文中，研究了低浓度姜黄素对人宫颈癌（HeLa）细胞的影响。我们发现姜黄素介导细胞数量和活力下降，凋亡事件和超氧化物水平增加。与之前显示的姜黄素对不同宫颈癌细胞系的细胞毒性相反，我们观察到即使使用低至1μM浓度的姜黄素也有毒性作用。姜黄素对HeLa细胞没有基因毒性。由于与正常细胞相比，恶性细胞中嗜银核仁蛋白（AgNOR蛋白）表达升高，反映了癌细胞增殖的速度，我们评估了姜黄素相关的银沉积大小（面积）和数量的变化。我们发现姜黄素诱导AgNOR蛋白池减少，这可能是由低浓度姜黄素处理后观察到的全基因组DNA高甲基化介导的。总之，我们首次表明低微摩尔浓度范围的姜黄素可能对HeLa细胞有效，这可能对基于姜黄素的人类宫颈癌治疗有影响。
