Martin Nicholas J, Cooper Helen J
School of Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK.
Expert Rev Proteomics. 2014 Dec;11(6):685-95. doi: 10.1586/14789450.2014.965158. Epub 2014 Oct 11.
Dried blood spots (DBS) have been used as a clinical sample format for over 50 years, and have been analyzed for small molecules and metabolites by mass spectrometry (MS) since the early 1990s. In the meantime, MS has become the tool of choice in proteomics. Despite this obvious avenue of scientific investigation, the marriage of MS and DBS protein analysis has been comparatively recent. DBS are a potentially rich source of protein biomarkers that remain to be exploited. This article focuses on the progress made in the mass spectrometric analysis of proteins from DBS and discusses the benefits and challenges facing this emerging field.
干血斑（DBS）作为一种临床样本形式已使用了50多年，自20世纪90年代初以来一直通过质谱（MS）对其进行小分子和代谢物分析。与此同时，质谱已成为蛋白质组学中的首选工具。尽管这是一条明显的科学研究途径，但质谱与干血斑蛋白质分析的结合相对较新。干血斑是蛋白质生物标志物的潜在丰富来源，仍有待开发。本文重点介绍了干血斑蛋白质质谱分析取得的进展，并讨论了这一新兴领域面临的益处和挑战。
