Pajic J, Rakic B, Jovicic D, Milovanovic A
Serbian Institute of Occupational Health "Dr Dragomir Karajovic", Deligradska 29, 11000 Belgrade, Serbia.
Singidunum University, Faculty of Applied Ecology (FUTURA), Pozeska 83a, 11000 Belgrade, Serbia.
Mutat Res Genet Toxicol Environ Mutagen. 2014 Oct;773:23-8. doi: 10.1016/j.mrgentox.2014.07.009. Epub 2014 Aug 7.
Biological dosimetry using chromosome damage biomarkers is a valuable dose assessment method in cases of radiation overexposure with or without physical dosimetry data. In order to estimate dose by biodosimetry, any biological dosimetry service have to have its own dose response calibration curve. This paper reveals the results obtained after irradiation of blood samples from fourteen healthy male and female volunteers in order to establish biodosimetry in Serbia and produce dose response calibration curves for dicentrics and micronuclei. Taking into account pooled data from all the donors, the resultant fitted curve for dicentrics is: Ydic=0.0009 (±0.0003)+0.0421 (±0.0042)×D+0.0602 (±0.0022)×D(2); and for micronuclei: Ymn=0.0104 (±0.0015)+0.0824 (±0.0050)×D+0.0189 (±0.0017)×D(2). Following establishment of the dose response curve, a validation experiment was carried out with four blood samples. Applied and estimated doses were in good agreement. On this basis, the results reported here give us confidence to apply both calibration curves for future biological dosimetry requirements in Serbia.
使用染色体损伤生物标志物进行生物剂量测定，在有或没有物理剂量测定数据的辐射过量暴露情况下，是一种有价值的剂量评估方法。为了通过生物剂量测定来估计剂量，任何生物剂量测定服务都必须有自己的剂量响应校准曲线。本文揭示了对14名健康男性和女性志愿者的血液样本进行辐照后获得的结果，以便在塞尔维亚建立生物剂量测定，并生成双着丝粒和微核的剂量响应校准曲线。考虑到所有捐赠者的汇总数据，双着丝粒的拟合曲线为：Ydic = 0.0009（±0.0003）+ 0.0421（±0.0042）×D + 0.0602（±0.0022）×D²；微核的拟合曲线为：Ymn = 0.0104（±0.0015）+ 0.0824（±0.0050）×D + 0.0189（±0.0017）×D²。在建立剂量响应曲线后，用4个血液样本进行了验证实验。应用剂量和估计剂量吻合良好。在此基础上，本文报告的结果让我们有信心将这两条校准曲线应用于塞尔维亚未来的生物剂量测定需求。
