Maldonado Andrés A, Cristóbal Lara, Martín-López Javier, Mallén Mar, García-Honduvilla Natalio, Buján Julia
Department of Plastic and Reconstructive Surgery and Burn Unit, University Hospital of Getafe, Madrid, Spain.
Department of Pathology, University Hospital of Puerta de Hierro, Madrid, Spain.
PLoS One. 2014 Oct 13;9(10):e109003. doi: 10.1371/journal.pone.0109003. eCollection 2014.
Pressure ulcers are a prevalent health problem in today's society. The shortage of suitable animal models limits our understanding and our ability to develop new therapies. This study aims to report on the development of a novel and reproducible human skin pressure ulcer model in mice.
Male non-obese, diabetic, severe combined immunodeficiency mice (n = 22) were engrafted with human skin. A full-thickness skin graft was placed onto 4×3 cm wounds created on the dorsal skin of the mice. Two groups with permanent grafts were studied after 60 days. The control group (n = 6) was focused on the process of engraftment. Evaluations were conducted with photographic assessment, histological analysis and fluorescence in situ hybridization (FISH) techniques. The pressure ulcer group (n = 12) was created using a compression device. A pressure of 150 mmHg for 8 h, with a total of three cycles of compression-release was exerted. Evaluations were conducted with photographic assessment and histological analysis.
Skin grafts in the control group took successfully, as shown by visual assessment, FISH techniques and histological analysis. Pressure ulcers in the second group showed full-thickness skin loss with damage and necrosis of all the epidermal and dermal layers (ulcer stage III) in all cases. Complete repair occurred after 40 days.
An inexpensive, reproducible human skin pressure ulcer model has been developed. This novel model will facilitate the development of new clinically relevant therapeutic strategies that can be tested directly on human skin.
压疮是当今社会普遍存在的健康问题。合适动物模型的短缺限制了我们对其的理解以及开发新疗法的能力。本研究旨在报告一种新型且可重复的小鼠人皮肤压疮模型的建立。
将人皮肤移植到22只雄性非肥胖、糖尿病、严重联合免疫缺陷小鼠身上。在小鼠背部皮肤上制造4×3厘米的伤口，然后植入全层皮肤移植物。60天后对两组永久性移植物进行研究。对照组（n = 6）着重于移植过程。通过摄影评估、组织学分析和荧光原位杂交（FISH）技术进行评估。压疮组（n = 12）使用压迫装置制造压疮。施加150 mmHg的压力，持续8小时，总共进行三个压迫 - 释放循环。通过摄影评估和组织学分析进行评估。
通过视觉评估、FISH技术和组织学分析表明，对照组的皮肤移植成功。第二组的压疮在所有病例中均表现为全层皮肤缺失，所有表皮和真皮层均有损伤和坏死（溃疡III期）。40天后完全修复。
已建立一种廉价、可重复的人皮肤压疮模型。这种新型模型将有助于开发新的临床相关治疗策略，这些策略可直接在人皮肤上进行测试。
