Chemical Engineering Department, Institute of Chemical Technology, Matunga, Mumbai 400019, India.
Chemical Engineering Department, Institute of Chemical Technology, Matunga, Mumbai 400019, India.
Bioresour Technol. 2014 Dec;173:342-351. doi: 10.1016/j.biortech.2014.09.108. Epub 2014 Oct 2.
In the present study, an attempt was made to evaluate the bacterial decolorisation of Reactive Blue 19 by an Enterobacter sp.F which was isolated from a mixed culture from anaerobic digester for biogas production. Phenotypic characterization and phylogenetic analysis based on DNA sequencing comparisons indicate that Enterobacter sp.F was 99.7% similar to Enterobacter cloacae ATCC13047. The kinetics of Reactive Blue 19 dye decolorisation by bacterium had been estimated. Effects of substrate concentration, oxygen, temperature, pH, glucose and glucose to microbe weight ratio on the rate of decolorisation were investigated to understand key factor that determines the performance of dye decolorisation. The maximum decolorisation efficiency of Reactive Blue 19 was 90% over period of 24 h for optimized parameter. To the best of our knowledge, this research study is the report where Enterobacter sp.F has been reported with about 90% decolorizing ability against anthraquinone based Reactive Blue 19 dye.
在本研究中，尝试用一株从沼气生产厌氧消化器混合培养物中分离出的肠杆菌属 F 来实现活性艳蓝 19 的细菌脱色。基于 DNA 测序比较的表型特征和系统发育分析表明，肠杆菌属 F 与肠杆菌 cloacae ATCC13047 的相似度为 99.7%。对细菌对活性艳蓝 19 染料的脱色动力学进行了估计。研究了底物浓度、氧气、温度、pH 值、葡萄糖和葡萄糖与微生物重量比等因素对脱色速率的影响，以了解决定染料脱色性能的关键因素。在优化参数的条件下，活性艳蓝 19 的最大脱色效率在 24 小时内达到 90%。据我们所知，这项研究是首次报道肠杆菌属 F 对蒽醌型活性艳蓝 19 染料具有约 90%的脱色能力。
